植物蛋白质组学研究若干重要进展

Research Advances in Plant Proteomics

植物蛋白质组学近年来正从定性向精确定量蛋白质组学的方向发展。国际上近两年发表的约160篇研究论文报道了利用不断改进的双向电泳结合生物质谱技术、多维蛋白质鉴定技术,以及包括双向荧光差异凝胶电泳、15N体内代谢标记、同位素标记的亲和标签、同位素标记相对和绝对定量等在内的第2代蛋白质组学技术,对植物组织(器官)与细胞器、植物发育过程和植物响应环境胁迫的蛋白质组特征,以及植物蛋白质翻译后修饰和蛋白质相互作用等方面的研究成果。该文对上述报道进行总结,综述了2007年以来植物蛋白质组学若干重要问题研究的新进展。

In recent years, plant proteomics have advanced from a qualitative to an accurate quantitative stage. Original research articles published in 2007 and 2008 numbered nearly 160. Most of them reported on the proteomic features of plant tissue （organs） and organelles, plant development, plant response to environmental stress, and post-translational modification and protein-protein interactions. These proteome analyses mainly involved continuously improved 2-D electrophoresis-mass spectrometry （2DE-MS）,multidimensional protein identification technology （MudPIT）, and second-generation proteomics techniques such as 2-D fluorescence difference gel electrophoresis （2-D DIGE）, 15N metabolic labeling, isotope-coded affinity tag （ICAT） and isobaric tags for relative and absolute protein quantitation （iTRAQ）. This paper gives an overview of the research advances of plant proteomics in the past two years.