He-Ne激光辐照与UV-B辐射对小麦幼苗细胞器中Na^+/K^+-ATP酶活性的影响

Effects of He-Ne Laser and UV-B Radiation on Na^+/K^+-ATPase Activity in Organelles of Wheat Seedlings

分别采用5mJ.s-1.mm-2He-Ne激光辐照、10.08kJ.m-2.d-1增强UV-B辐射及二者组合对小麦(Triticum aestivum)晋麦8号(Triticum aestivum‘Jinmai8’)幼苗进行处理。第5天开始测定各处理小麦幼苗叶片中线粒体、叶绿体及细胞溶质中Na+/K+-ATP酶活性的变化。结果表明,随着处理天数的增加,小麦幼苗叶片线粒体、叶绿体及细胞溶质中Na+/K+-ATP酶活性均在第6天下降,第7天升高,而后又逐渐下降。在处理的第7天,仅He-Ne激光辐照可使小麦幼苗叶片线粒体、叶绿体及细胞溶质中Na+/K+-ATP酶活性升高;增强UV-B辐射使各细胞器中Na+/K+-ATP酶活性下降;复合处理后小麦各细胞器中Na+/K+-ATP酶活性均高于UV-B单独辐射处理。实验结果表明,一定剂量的He-Ne激光辐照能够部分修复UV-B辐射对小麦幼苗细胞器中Na+/K+-ATP酶造成的损伤。

Seedlings of wheat （Triticum aestivum） were exposed to He-Ne laser irradiation with 5mJ·s^-1·mm^-2 power density, enhanced UV-B radiation （10.08 kJ·m·^-2,d^-1） and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation.From day 5, changes of Na^＋/K^＋-ATPase activity in mitochondria, chloroplasts and cellular solutes were measured to determine the effects of He-Ne laser irradiation and UV-B radiation on wheat.Na^＋/K^＋-ATPase activity in mitochondria, chloroplasts and cellular solutes of wheat seedlings was reduced at day 6, increased at day 7, then gradually decreased with treatment days. At day 7, He- Ne laser irradiation resulted in increased activity of Na^＋/K^＋-ATPase in mitochondria, chloroplasts and cellular solutes of wheat seedlings. The activity of Na^＋/K^＋-ATPase was reduced under UV-B radiation. The Na^＋/K^＋-ATPase activity in various organelles by combined treatments was greater than the activity with UV-B radiation. All these results suggested that damage to wheat seedlings induced by enhanced UV-B radiation in terms of activity of Na^＋/K^＋-ATPase in various organelles can be repaired in part by He-Ne laser irradiation.