25-羟维生素D参考区间的建立及其临床意义

Establishment of 25-OH-vitamin D reference interval by radioimmunoassay and its clinical significance

目的建立区域人群25-羟维生素D（25-OH-Vit D）的参考区间，并对用于临床评估提出建议。方法收集204例健康参考个体的样本，用放射免疫法进行检测，对检测结果进行离群点检查、绘制数据分布图，统计分析25-OH-VitD的检测结果，对参考区间进行验证和建立。结果建立的25-OH-vitD参考区间青少年为16．0～39．7ng／ml，成年人为11．7～40．0ng／ml。结论25-OH-VitD在人体水平因各个地区人群的年龄、性别、生活习惯等的差异而有所不同。国内区域人群维生素D的摄人量可能整体偏低，故临床医生在评估维生素D状态时，应考虑人体所应有的足够量的25-OH-Vit D。

Objective To establish reference interval of 25-OH-vitamin D （25-OH-Vit D） by radioimmunoassay and provide suggestion for clinical applications. Method Collecting 204 healthy persons specimens, and validating and establishing reference interval of 25-OH-Vit D by treatment of outlying observations, judgement of data distributions and analysis of test results. Results The reference interval of 25-OH-Vit D established in our laboratory is 16.0~39.7 ng/ml for adolescents and 11.7~40.0 ng/ml for adults. Conclusions The levels of 25-OH-Vit D in humans depend on their age, sex and life style. Some people＇s vitamin D intake is not enough. Doctors should pay attention to the sufficient 25-OH-Vit D needed when evaluate vitamin D intake.