不同激素配伍对雨生红球藻(Haematococcus pluvialis)细胞生长和虾青素累积的调节作用、藻株差异及应用

CELL GROWTH AND ASTAXANTHIN ACCUMULATION OF FOUR STRAINS OF HAEMATOOCCUS PLUVIALIS EXPOSED TO DIFFERENT PLANT REGULATORS

利用生长素(NAA、3-IBA)和细胞分裂素(6-BA、kt)两类植物激素,对比研究了其不同配伍对4株雨生红球藻H0、H2、H3和H4细胞生长以及虾青素累积的调节作用。结果表明,尽管在不同株系之间存在一定的差异,但激素明显促进该藻细胞生长和虾青素累积,而对细胞大小没有产生明显影响。激素对红球藻细胞数量的增加主要是靠加快游动细胞阶段无性繁殖过程来实现的;而激素对虾青素含量的提高却是通过增加细胞数量和细胞内虾青素积累的协同作用结果,其中以细胞数量的增加为主。在激素单因素实验中,3-IBA对H0细胞生长增加效果最好,对H2、H3细胞生长增加效果较好的是kt,对H4细胞生长增加效果最明显的是NAA。与空白对照组相比,上述激素处理后细胞生长速度分别提高了1.95、1.54、4.25和1.78倍。经过激素多因素实验,H0、H2、H3和H4藻株的细胞生长增加效果分别是空白组的1.44、2.62、2.52、1.07倍,而虾青素含量分别为空白组的1.09、1.61、1.37、2.35倍。根据4株藻株的实验结果以及综合考虑各激素的市场价格,建议在规模化生产中,采用1.75mg/L NAA和4mg/L 3-IBA以提高红球藻的培养效果。

The regulatory effect of different combinations of plant regulators [auxin （NAA, 3-IBA） and cytokinin （6-BA, kt）] on cell growth and astaxanthin accumulation in four strains of Haematococcus pluvialis were compared in an orthogonal experimental design. The results show that using phytohormones at a proper concentration can significantly increase cell growth and astaxanthin accumulation. The increase in cell number is realized by accelerating the asexual propagation in the motile cell stage, while that in astaxanthin accumulation depends on mainly cell growth rate and partly on high astaxanthin content in cell. In a single-factor experiment, among plant regulators, 3-IBA is shown the best for H0 cell growth, kt for promoting cell growth of strains H2 and H3, and NAA for H4 growth. The cell growth rate increase by 1.54-4.25 times over that of control. In a multiple-factors experiment, the cell numbers is 1.07-2.62 times higher than its control group, so does the astaxanthin accumulation amount for 1.09-2.35 times higher. Considering the current market price of phytohormones, NAA at 1.75mg/L and 3-IBA at 4mg/L are recommended in mass cultivation of H. pluvialis.