天麻化学成分研究(Ⅲ)

Chemical constituents from Gastrodia elata(Ⅲ)

目的研究天麻中的强极性成分。方法大孔吸附树脂柱结合制备液相色谱分离、光谱分析及理化常数比较鉴定结构。结果从天麻大孔吸附树脂水洗脱部位及20%乙醇洗脱部位分离并鉴定了10个化合物,分别为柠檬酸单乙酯(Ⅰ)、尿苷(Ⅱ)、腺嘌呤(Ⅲ)、肿根糖A(dactylose A,Ⅳ)、尿嘧啶(Ⅴ)、酪氨酸(Ⅵ)、N2-(对羟苄基)-鸟苷(Ⅶ)、1-异阿魏酸-β-D-吡喃葡萄糖苷(Ⅷ)、4-(β-D-吡喃葡萄糖氧)苯甲醛(Ⅸ)、对羟基苯甲酸(Ⅹ)。结论化合物Ⅶ为新化合物,命名为天麻核苷(gastronucleoside),其他9个化合物均为天麻首次报道成分。

Objective To study the high polar constituents of Gastrodia data. Methods The constituents were isolated by macroporous resin column chromatography combined with preparative HPLC and identified by spectral analyses and physico-chemical properties comparisons. Results Ten compounds were isolated from H2O and 20% EtOH fractions eluted from resin column and their structures were identified as citric acid mono-ethyl ester （ Ⅰ ）, uridine （ Ⅱ ）, adenine （ Ⅲ ）, dactylose A （Ⅳ）, uracil （ Ⅴ ）, tyrosine （Ⅵ）, N2-（p-hydroxybenzyl） guanosine （ Ⅶ ）, l-isoferuloyl-β-D-glucopyranoside （ Ⅷ ）, 4-β-D-glucopyranosyloxy） benzaldehyde （Ⅸ）, and p-hydroxyl benzoic acid （ Ⅹ ）. Conclusion Compound Ⅶ is a new compound named gastronucleoside. Other nine compounds are isolated from G. elata for the first time.