棉花黄萎病拮抗细菌2-70(Bacillus subtilis)菌株定殖能力的研究

Studies on colonization of Antagonistic Bacteria 2-70(Bacillus subtilis) to Cotton Verticillium dahliae

试验通过灭菌土和非灭菌土两种土样,试验前分别对这两种土样进行三种不同的处理,即:用拮抗菌芽孢液浸种,土壤全部混菌,部分混菌进行盆栽试验。主要通过盆栽试验,考察棉花黄萎病拮抗细菌2-70在土壤中的定植情况及在棉花根内和根际的定植情况。结果表明,在实验室条件下,拮抗细菌2-70能够在灭菌土和非灭菌土中定殖,且定殖数量达106cfu/g土左右,经过五个月后仍能保持较高的抑菌活性,并且菌株在灭菌土中的定殖数量高于非灭菌土中的数量。通过对棉苗根内细菌的回收,证实拮抗细菌2-70能在棉花根内定殖,数量达到103cfu/g。

This test required sterile soil and non-sterile soil in two soil samples, test separately for the two soil samples of three different treatments that is： The antagonistic bacterium Bacillus soaking liquid, soil all mixed soil and some soil for potted plants mixed. In this paper, a pot experiment to study Verticillium dahliae 2-70 antagonistic bacteria in the soil and in the colonization of cotton roots and rhizosphere colonization of the situation. The results showed that under laboratory conditions, antagonistic bacteria can 2-70 in non-sterilized soil and sterilized soil colonization, and colonization Tatsu quantity 10^6 cfu/g soil around, after five months has managed to maintain a higher antibacterial activity, and strains in sterile soil in the quantity colonization than non-sterilized soil in quantity. Cotton roots by bacteria recovery, confirmed the antagonistic bacteria in the 2-70 designated cotton root colonization, number of 10^3 cfu/g.