摘要
利用从藏灵菇中筛选的马克斯克鲁维酵母M3菌株探讨胃肠道耐受性及体外降胆固醇功效。分别在pH1.5~4.5、胆盐浓度0.3%~3.0%、NaCl浓度3.0%-6.0%的模拟胃肠道环境下,采用活菌计数法检测M3菌株的耐胃肠道逆环境特性,以邻苯二甲醛法检测其降胆固醇效果。结果表明:在pH1.5-4.5作用4h活菌数可达10^6cfu/ml,0.3%~3.0%胆盐及3.0%~6.0%NaCl作用8h活菌数可达10^5-10^6cfu/ml,在pH1.5~3.0(4h)及胆盐浓度0.3%~3.0%的模拟人体胃肠道环境下降胆固醇率均在60%以上。显示M3菌株具有较强的耐胃肠道逆环境特性及良好的降胆固醇能力,为开发研制降胆固醇功能食品及微生态制剂提供了试验基础。
Using Kluyveromyces marxianus M3 isolated from Tibetan Kefir to study tolerance characteristics of the gastrointestinal tract and cholesterol-lowering effects in vitro. Viable count was used to detect M3 stains' tolerance characteristics of the gastrointestinal tract separately under simulated gastrointestinal tract environment pH 1.5-4.5, salt concentration 0.3%-3.0%, sodium chloride 3.0%-6.0%. At the same time, using O-phthalaldehyde to detect the effect of its cholesterol-lowering. The result shows that after 4 hours under pH 1.5-4.5 the viable can get 10^6 cfu/ml. The viable can be 10^5-10^6 cfu/ml under 0.3%-3.0% bovine bile salt and 3.0%-6.0% sodium chloride (8 h). Also, in the simulated gastrointestinal tract environment pH 1.5-3.0 (4 h), salt concentration 0.3%-3.0% (8 h) the cholesterol-lowering can reach 60%. M3 strains have strong tolerance characteristics against the environment of the gastrointestinal tract and the good cholesterol-lowering ability, which provide the experimental basis for the development of cholesterol-lowering functional foods and probiotics' study.
出处
《中国农学通报》
CSCD
北大核心
2009年第15期60-63,共4页
Chinese Agricultural Science Bulletin
基金
北京市自然科学基金资助项目"藏灵菇克鲁维酵母菌降胆固醇作用及机理的研究"(5092008)
农产品加工及贮藏工程北京市重点建设学科项目(PXM2009_014207_078172)
关键词
马克斯克鲁维酵母
耐受性
胃肠道环境
降胆固醇
Kluyveromyces marxianus, tolerance characteristics, gastrointestinal tract, cholesterol-lowering