光学微扫描显微热成像扫描零点定标方法研究

Zero Calibration for the Designed Microscanning Thermal Microscopic Imaging System

为改善已研制光学微扫描显微热成像系统的空间分辩力,微扫描零点需要确定。基于几何原理,研究提出了一种利用数字图像处理技术进行零点定标的方法。给出了微扫描零点的定义、详细分析了零点定标原理及方法,完成了实际显微热成像系统的微扫描零点定标。针对红外热图像,模拟零点定标前后的实际系统,采用不同重构方法进行了仿真研究,给出了评价参数;利用零点定标后的光学微扫描显微热成像系统采集低分辨力显微热图像序列进行过采样重构研究,仿真和实验结果表明了该方法的有效性,从而得到了高分辨力光学微扫描显微热成.像系统,可应用于需要显微热分析的场合。

In order to improve the spatial resolution of the optical microscanning thermal microscopic system, the zero of microscanning should be determined to complete the oversample reconstruction. Based on the geometric principle and digital imaging processing, a new zreo calibration was presented. The definition, the working principle and the method were analyzed, and the microscanning zero calibration of the real system was done. Different reconstruction methods were applied to reconstruct the thermal image and the real thermal microscopic image, moreover the evaluation parameters were given. Results of simulation and real thermal imaing peocessing show the availability of the zero calibration, thus the high resolution optical microscanning thermal microscopic system has been achieved. The system can be applied into many systems which need microscopic thermal anslysis with high spatial resolution.