肝癌细胞和正常肝细胞的自体荧光光谱研究

Study of Autofluorescence Spectrum between Hepatic Carcinoma Cell and Hepatic Cell

利用荧光光纤传感器来检测癌症必须弄清楚肝癌细胞与正常肝细胞是否具有不同的荧光特征。在体外培养了SMMC-7721肝癌细胞和HL-7702正常肝细胞,然后用荧光光谱仪对它们的自体荧光进行检测。结果表明,两种细胞的自体荧光发射光谱在550～720 nm范围内发射峰的数目和峰位均相同(602 nm和691 nn),只是主峰的波形略有差异。在720～800 nm范围内,肝癌细胞有一特征荧光峰,峰位置在734 nm附近,而正常肝细胞则无此峰。通过对多代培养的细胞进行荧光光谱测试,发现两种细胞的荧光光谱特征在细胞传代的过程中保持不变,说明细胞中的荧光特征在传代过程中保持稳定。这两方面的实验为利用荧光光纤传感器来检测癌症提供了一定的参考。

The objective of the autofuorescence spectrum of hepatic carcinoma cell and hepatic cell studied was to understand if there was difference between the two cells. So we cultured SMMC-7721 hepatic carcinoma cell and HL- 7702 hepatic cell in vitro. Then their autofuorescence was detected by fluorescent spectrograph. It presents that the spectrogram of the hepatic carcinoma cell resembles that of the hepatic cell at the range of wavelength from 550-720 nm characterized by two peaks at 602 nm and 691 nm. But from 721-800 nm, the spectra of carcinoma cell with a peak located at 734 nm were found. For the hepatic cell the peak at 734 nm was not observed. The experimentations of propagation of both cells confirm ulteriorly that there is a significant difference between two cells＇spectrum. The experiment will be beneficial to the next research of detecting hepatic carcinoma by fluorescent fiber optic biosensor.