利血平对大鼠唾液蛋白分泌的影响

Effect of reserpine on the secretion of salivary protein in rats

目的:观察利血平对大鼠唾液蛋白分泌的影响.方法:实验大鼠随机分为2组,实验组皮下注射利血平0.4mg/(kg·d),正常组皮下注射等量生理盐水.10d后,抽取唾液检测酸刺激前后唾液淀粉酶活性(sAA)比值;HE染色检测腮腺形态学和透射电镜计数腮腺酶原颗粒;ELISA法检测血管活性肠肽(VIP)和环磷酸腺苷(cAMP)含量.结果:实验组较正常组摄食量明显减少,体质量下降;sAA活性比值显著低于正常组(0.39±0.18vs0.80±0.21,P<0.01);HE染色未见2组腮腺有明显病理改变,透射电镜发现实验组腮腺单位观察视野内酶原颗粒数量明显高于正常组(41.4±4.9vs34.6±5.2,P<0.01);实验组血清VIP含量明显低于正常组(22.5±13.1μg/Lvs38.5±14.1μg/L,P<0.05),2组腮腺VIP含量无明显差异;实验组血清cAMP明显高于正常组(125.8±15.5nmol/Lvs105.3±16.7nmol/L,P<0.05);2组腮腺cAMP水平无明显差异.结论:利血平导致大鼠消化代谢机能下降,在酸刺激下大鼠的唾液蛋白分泌明显减少,可能是通过降低血清VIP水平调节唾液蛋白分泌.

AIM： To observe the effect of reserpine on the secretion of salivary protein in rats. METHODS： All the rats were divided into experimental group and normal control group. Rats in the experimental group received subcutaneous injection of reserpine [0.4 mg/（kg·d）] while rats in the control group received the same volume of saline instead. After 10 days＇ treatment, salivary samples were collected from rats to detect the ratio of salivary amylase （sAA） before and after acid stimulation. Zymogen granules were counted under transmission electron microscopy. The content of vasoactive intestinal peptide （VIP） and cyclic adenosine monophosphate （cAMP） in the serum and parotid tissues were assayed using ELISA. RESULTS： Food intake was significantly decreased and weight was reduced in the experimental group compared with the normal group. The ratio of sAA in the experimental group was significantly lower than the normal group （0.39 ± 0.18 vs 0.80 ± 0.21, P 〈 0.01）. No difference in pathological changes was found between the two groups, and the number of zymongen granules under transmission electron microscopy was remarkably higher in experimental group than the normal group （41.4 ± 4.9 vs 34.6 ± 5.2, P 〈 0.01）. The content of VIP in serum was lower in the experimental group than the normal group （22.5 ± 13.1 μg/L vs 38.5 ± 14.1 μg/L, P 〈 0.05）. No difference was noted in content of VIP in parotid tissues between the two groups, and the serum cAMP was remarkably higher in the experimental group than in the normal group （125.8 ± 15.5 nmol/L vs 105.3 ± 16.7 nmol/L, P 〈 0.05） but no difference was found in the content of cAMP in parotid tissues between the two groups CONCLUSION： Reserpine decreases the digestive and metabolic function in rats; the secretion of salivary protein in rats is decreased significantly after the stimulation of acid, which regulates the secretion of salivary protein possibly though reducing the level of VIP in serum.