一种原代培养大鼠DRG神经元的新方法

A new method for primarily culturing DRG neurons of rats

目的建立并提供一种可供客观衡量和评价实验研究结果的稳定、高效的体外原代神经细胞的培养模型。方法采用神经细胞专用无血清培养基——Neurobasal作为培养介质,用喜树碱纯化DRG神经元培养体系,通过对背根神经节分离、细胞培养以及纯化等多个环节和步骤的比较研究,确定了DRG神经元原代培养和纯化的最佳条件和步骤。结果采用上述方法,可获得具有良好活性的高纯度原代DRG神经元(>98%)。结论该培养体系稳定可靠、适用于生物学和药理学等相关的体外实验研究。

Aim To establish and provide a highly efficient and stable in vitro primary culture model of neuronal cells used for the objective assessment and evaluation of experimental results. Methods Using the serum-free neurohasal specific for culture of neuronal cells as culture medium and CPT as a reagent to eliminate non-neuronal proliferating ceils; the culture conditions as well as steps for isolating and purificating DRG neurons were optimized through comparative studies among different experimental conditions. Results High viability and purity DRG neurons can be obtained by taking the methods mentioned above. Conclusion The primary culture model of DRG neurons was stable and reliable and suitable for the study of biological and pharmacological in vitro experiments.