B7-H4基因对SKOV3细胞生长功能的影响

Effect of B7-H4 gene transfection on growth of ovarian cancer cells

目的:初步探讨B7-H4基因对人卵巢癌细胞株SKOV3细胞生长和致瘤性的影响。方法:RT-PCR法扩增编码基因,构建真核表达载体PEGFP-N1/B7-H4,以脂质体为载体将重组质粒PEGFP-N1和PEG-FP-N1/B7-H4分别导入SKOV3细胞中,筛选建立高表达的细胞株。MTT法绘制体外培养细胞生长曲线,将转染前后的肿瘤细胞分别接种于SCID小鼠的腹部皮下观察其致瘤性。结果:成功构建了人B7-H4真核表达载体,SK-OV3/B7-H4细胞高表达B7-H4,转染后肿瘤细胞体外生长速度明显增快。SKOV3/B7-H4细胞在小鼠体内的致瘤性较SKOV3/neo细胞和野生型SKOV3细胞明显升高。结论:B7-H4基因导入细胞后在体外和体内具有明显加快肿瘤生长的作用,可作为肿瘤基因治疗的靶向基因。

AIM ： To observe the effect of B7 - H4 gene transfection on human ovarian cancer cell growth and its tumor formation. METHODS ： Human ovarian cancer cell line SKOV3 was transfected with PEGFP - N1/B7 - H4 and PEGFP - N1 by LipofectamineTM2000 method. The expression of B7 - H4 gene was detected by RT - PCR. The high expression cell strain was selected. The growth curve was drawn by MTT methods. The tumor size was observed after SKOV3/ B7- H4 cells, SKOV3/neo cells and SKOV3 cells were injected subcutaneously into SCID mouse. RESULTS： The expressions of B7 - H4 mRNA and fusion protein in SKOV3/B7 - H4 cells were positive. Compared with the other two groups, transfection significantly promoted cell proliferation in vitro. In addition , facilitated tumor formation and enhanced tumor growth were observed in SKOV3/B7 - H4 group. No difference in tumor growth between SKOV3 group and SKOV3/ N1 group was observed. CONCLUSION： B7 -H4 may be a candidate gene for gene therapy of ovarian cancer.