摘要
目的:观察趋化因子fractalkine(CX3CL1,Fkn)对人单核细胞株U937细胞基质金属蛋白酶-2(MMP-2)表达与分泌的影响。方法:使用不同浓度的人重组Fkn干预U937细胞,并使用人肾系膜细胞(HRMC)上清及Fkn中和抗体干预U937细胞,RT-PCR法检测细胞MMP-2mRNA表达,明胶酶谱法检测细胞上清中MMP-2水平。结果:不同浓度人重组Fkn干预后U937细胞MMP-2的表达及上清中MMP-2水平低于空白对照组,HRMC上清干预组MMP-2表达水平低于空白对照组,而HRMC上清加Fkn中和抗体干预组MMP-2表达水平及上清中MMP-2水平明显高于单用HRMC上清干预组。结论:Fkn可抑制U937细胞MMP-2的表达与分泌,而HRMC可能通过Fkn影响U937细胞MMP-2的表达与分泌。
AIM: To study the effect of fractalkine (CX3CL1, Fkn) on the expression and secretion of matrix metalloproteinase- 2 (MMP- 2) in cultured human monocyte line U937 cells. METHODS: The cultured U937 cells were incubated with recombinant human Fkn, the supernatant of human renal mesangial cells (HRMC) and Fkn neutralizing antibodies for 24 h. The mRNA expression of MMP - 2 was analyzed by RT - PCR. The production of MMP - 2 in the supernatant was analyzed by gelatin zymography. RESULTS : The level of MMP - 2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group. Similarly, the level of MMP -2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group. However, the level of MMP- 2 mRNA and protein in the ceils incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC. CONCLUSION: Fkn inhibits the expression and secretion of MMP -2 in cultured U937 cells. HRMC might mediate the expression and secretion of MMP -2 in U937 cells through Fkn.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第8期1621-1624,共4页
Chinese Journal of Pathophysiology
基金
教育部留学归国人员科研启动基金资助项目(No.6890000001)