Fractalkine对人单核细胞MMP-2的表达及分泌的影响

Effect of fractalkine on the expression and secretion of MMP-2 in human monocytes

目的:观察趋化因子fractalkine(CX3CL1,Fkn)对人单核细胞株U937细胞基质金属蛋白酶-2(MMP-2)表达与分泌的影响。方法:使用不同浓度的人重组Fkn干预U937细胞,并使用人肾系膜细胞(HRMC)上清及Fkn中和抗体干预U937细胞,RT-PCR法检测细胞MMP-2mRNA表达,明胶酶谱法检测细胞上清中MMP-2水平。结果:不同浓度人重组Fkn干预后U937细胞MMP-2的表达及上清中MMP-2水平低于空白对照组,HRMC上清干预组MMP-2表达水平低于空白对照组,而HRMC上清加Fkn中和抗体干预组MMP-2表达水平及上清中MMP-2水平明显高于单用HRMC上清干预组。结论:Fkn可抑制U937细胞MMP-2的表达与分泌,而HRMC可能通过Fkn影响U937细胞MMP-2的表达与分泌。

AIM： To study the effect of fractalkine （CX3CL1, Fkn） on the expression and secretion of matrix metalloproteinase- 2 （MMP- 2） in cultured human monocyte line U937 cells. METHODS： The cultured U937 cells were incubated with recombinant human Fkn, the supernatant of human renal mesangial cells （HRMC） and Fkn neutralizing antibodies for 24 h. The mRNA expression of MMP - 2 was analyzed by RT - PCR. The production of MMP - 2 in the supernatant was analyzed by gelatin zymography. RESULTS ： The level of MMP - 2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group. Similarly, the level of MMP -2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group. However, the level of MMP- 2 mRNA and protein in the ceils incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC. CONCLUSION： Fkn inhibits the expression and secretion of MMP -2 in cultured U937 cells. HRMC might mediate the expression and secretion of MMP -2 in U937 cells through Fkn.