HPLC法测定尿免疫化学非反应性白蛋白及其在早期诊断儿童糖尿病肾病中的意义

Determination of immunochemically nonreactive urinary albumin by HPLC method and its significance of diagnose on children diabetic nephropathy

目的:研究尿免疫化学非反应性白蛋白的检测对早期诊断儿童糖尿病肾病的意义,探讨HPLC法测定尿白蛋白的应用价值。方法:收集58份糖尿病患儿的随机尿标本,分别用高效液相色谱法(HPLC)和免疫比浊法对其进行微量白蛋白检测。结果:标准品和尿液标本的白蛋白出峰时间均为14.3min,58例糖尿病患儿尿液中,由HPLC法和免疫比浊法测定的尿白蛋白分别为9.6-195.1mg/L和0-158.6mg/L,在每份标本中HPLC法的测定结果均高于免疫比浊法(P<0.05)。HPLC法检出的微量白蛋白尿41例(70.7%),免疫比浊法检出22例(37.9%)。结论:HPLC法能检出全部的尿白蛋白,比免疫化学法检测出更多的尿微量白蛋白,可更早期诊断糖尿病患儿是否伴有持续性白蛋白尿。

AIM： To detect the immunochemically nonreactive urinary albumin and its significance of diagnose on diabetic nephropathy by high performance liquid chromatography （HPLC）. METHODS ： Urine samples of 58 diabetic children were collected. Urinary albumin was measured by HPLC and by immunoassay respectively. RESULTS： The retention times of albumin of standard and urine samples were 14. 3 min. The content of albumin by HPLC and by immunoassay were 9. 6 - 195. 1 mg/L and 0 - 158.6 mg/L, respectively. The level of urinary albumin was higher by HPLC detection than that by immunoassay （P 〈 0. 05 ） in the same sample. The microalbuminuria rates were 70.7% detected by HPLC and 37. 9% by immunoassay. CONCLUSION： Total urinary albumin can be measured by HPLC. Microalbuminuria in diabetic children can be observed earlier by HPLC.