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Thy-1及Thy-1mRNA在肺纤维化大鼠肺组织中的表达及相关性研究

The expression of Thy-1 and Thy-1mRNA and the correlation analysis in the animal models of lung frbrosis
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摘要 目的:以博莱霉素(BLM)致肺纤维化大鼠为动物模型,研究胸腺细胞分化抗原-1(thymocytedif-ferentiationantigen1,Thy-1/CD90)及Thy-1mRNA的表达,分析Thy-1及Thy-1mRNA与肺纤维化形成之间的关系,为研究肺纤维化的发病机制提供新的思路。方法:将大鼠随机分为实验组及对照组,用BLM气管注入法建立动物模型;分别留取第1d、3d、7d、14d、28d肺组织,测定羟脯胺酸(HYP)、Thy-1、Thy-1mRNA的表达情况。结果:正常肺间质中成纤维细胞多数表达Thy-1,阳性率为88.01%+4.12%;随着纤维化程度加重,Thy-1表达呈进行性减少;Thy-1表达阳性率与HYP之间呈负相关;Thy-1mRNA在肺纤维化形成过程中无明显变化,与Thy-1表达之间无相关性。结论:在肺纤维化形成过程中存在成纤维细胞表达Thy-1减少,Thy-1细胞可能是胶原的主要来源细胞;Thy-1的减少与Thy-1mRNA无关。
作者 张晓荣 吕长俊 王晓芝 崔剑 高福泉 郝东 王涛 李洪波
机构地区 滨州医学院附属医院呼吸内科 山东省立医院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2009年第8期1640-1643,共4页 Chinese Journal of Pathophysiology
基金 山东省卫生厅基金资助项目(No.2007QZ001)
关键词 Thy-1/CD90 肺纤维化 Thy - 1/CD90 Lung fibrosis
分类号 R363 [医药卫生—病理学]
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参考文献17

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二级参考文献16

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共引文献23

中国病理生理杂志
2009年 第8期

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