铕(Ⅲ)牛磺酸席夫碱配合物的合成、表征及其与DNA的作用模式

Synthesis,Characterization and Interaction Mode of Complexes of Europium(Ⅲ) and Taurine Schiff Base with DNA

合成了3种铕(Ⅲ)与牛磺酸缩水杨醛席夫碱配合物[(Eu(TSal)L(NO3)].2H2O(TSal:席夫碱配体,L1:1,10-邻菲咯啉,L2:2-氨基吡啶,L3:2,2′-联吡啶),并对其进行了结构表征;采用荧光光谱法和粘度法研究了配合物与小牛胸腺DNA的相互作用模式。实验结果表明,3种配合物对DNA均有不同强度的插入作用,其插入能力在一定范围内与配合物的浓度正相关,且与配合物分子适宜的平面性有关,3种配合物对DNA插入能力顺序为:[Eu(TSal)L1(NO3)]·2H2O>[Eu(TSal)L3(NO3)]·2H2O>[Eu(TSal)L2(NO3)]·2H2O。

Three complexes of europium（Ⅲ） with Taurine-salicylaldehyde Schiff base（[（Eu（TSal）L（NO3）]·2H2O,TSal：Taurine-salicylaldehyde Schiff base,L^1：1,10-phenanthroline,L^2：2-aminopyrimidine,L^3：2,2′-bipyridine）have been synthesized and characterized.The interaction mode between the complexes and calf thymus DNA has been investigated by means of fluorescence spectroscopy and viscosity measurement.The results show that all the complexes can remarkably quench the emission intensity of the EB-DNA system.With increasing the concentration of the complexes,the ability of their binding to DNA is more prominent under the experimental conditions.All the experimental results indicate that the interaction of the complexes with calf thymus DNA is an intercalation mode.The sequence of intercalating ability of the complexes is [Eu（TSal）L^1（NO3）]·2H2O〉[Eu（TSal）L^3（NO3）]·2H2O〉[Eu（TSal） L^2（NO3）]·2H2O.