甘草酸二铵与牛血清白蛋白相互作用的光谱

Spectroscopic Studies on the Interaction between Diammonium Glycyrrhizinate and Bovine Serum Albumin

在模拟人体生理条件下,用常规荧光光谱和紫外-可见吸收光谱研究了甘草酸二铵与牛血清白蛋白之间的相互作用,并利用同步荧光光谱及三维荧光光谱探讨了甘草酸二铵对牛血清白蛋白构象的影响。荧光光谱表明,甘草酸二铵对牛血清白蛋白具有荧光猝灭作用。根据不同温度下的荧光猝灭数据和紫外示差图谱证实,甘草酸二铵对BSA的荧光猝灭为动态猝灭过程。根据F rster非辐射能量转移理论计算了甘草酸二铵与牛血清白蛋白相互作用时,其给体-受体间的最近距离(r=2.28 nm)和能量转移效率(E=0.05);研究了常温下几种金属离子对该相互作用体系的影响。

The interaction between diammonium glycyrrhizinate（DG） and bovine serum albumin（BSA） was studied in physicological solution by spectroscopic methods including fluorescence spectrometry and UV-Visible absorption spectrometry.Synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the conformational change of BSA due to the addition of DG.Fluorescence titration reveals that the flurescence intensity of BSA at 348 nm was quenched when DG was added.The dynamic quenching existed between DG and BSA according to the flurescence quenching data at different temperatures and the UV differential spectra.According to Frster theory of non-radiation energy transfer,the nearest distance（r=2.28 nm） and transfer efficiency of energy（E=0.05） between donor（BSA） and acceptor（DG） were determined.The effects of metal ions on the system were studied at room temperature.