4种动物药水提液中3种嘌呤碱HPLC法测定

Determination of Hypoxanthine,Xanthine and Adenine Extracts in Four Animal Drugs by HPLC

以甲醇/水为流动相,反相高效液相色谱-紫外检测器测定了乌蛇、蟾蜍、土虫、地龙水提取液中黄嘌呤、次黄嘌呤、腺嘌呤的含量,对样品提取和高效液相色谱条件进行了优化。黄嘌呤、次黄嘌呤、腺嘌呤的检出限分别为0.012×10-6、0.041×10-6、0.042×10-6g/mL,样品分析的日内测定相对标准偏差分别小于4.1%、7.3%、4.5%,日间测定相对标准偏差分别小于5.8%、2.9%、3.1%,平均加标回收率为94%～107%。

Hypoxanthine,xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector.This process of sample preparation was improved and the conditions of HPLC were also optimized.The detection limits of hypoxanthine,xanthine and adenine were 0.012×10^-6,0.041×10^-6,0.042×10^-6 g/mL,respectively.The intra-day precisions（RSD） of hypoxanthine,xanthine and adenine were less than 4.1%,7.3% and 4.5%,respectively.The inter-day precisions（RSD） of hypoxanthine,xanthine and adenine were less than 5.8%,2.9% and 3.1%,respectively.The recovery rates of the four samples with added specimen were in a range of 94% to 107%.