灿烂甲酚蓝体系分光光度法测定肌苷

Spectrophotometric Determination of Inosine with Brilliant Cresyl Blue

在pH=6.9的BR缓冲体系中,灿烂甲酚蓝与肌苷反应,形成离子缔合物,溶液颜色发生改变。其最大褪色波长位于630 nm处,在褪色波长处,肌苷的浓度与溶液的褪色呈良好的线性关系,可用于肌苷的测定。肌苷的浓度在0.18×10-6～6.0×10-6g/mL范围内符合比尔定律,相关系数r=0.999 3,摩尔吸光系数为1.13×104L/(mol.cm),检出限为53.5×10-9g/mL。方法简便、准确、灵敏,可用于片剂中肌苷的测定。

In a pH=6.9 BR buffer medium,brilliant cresyl blue reacted with inosine to form ion-association complex.The maximum fading wavelength was located at 630 nm.The fading intensity is proportional to the concentration of inosine.The concentration of inosine in a range of 0.18×10^-6-6.0×10^-6 g/mL obeys Beer′s Law.The correlation coefficient is 0.999 3,the molar absorptivity is 1.13×10^4 L/（mol·cm）,the detection limit is 53.5×10^-9 g/mL.The method is simple,accurate and sensitive,and it was applied to the determination of inosine in tablet samples with satisfactory results.