转化生长因子β_1与雌激素在骨质疏松大鼠中的表达

Expression of transforming growth factor-β_1 and estrogen in osteoporosis rats expression

目的:建立并完善骨质疏松症(OP)动物模型,观察SD大鼠OP模型血清学中雌激素(E2)和关节骨组织中转化生长因子β1(TGF-β1)的表达,并观察它们之间的关系。方法:20只4个月龄雌性SD大鼠,随机分为空白组、OP模型组(采用摘除双侧卵巢法),每组10只。2个月后处死各组大鼠,取右股骨近端利用双能X线骨扫描仪进行骨密度检测,取左股骨髁关节骨组织行光镜组织形态学观察,酶联免疫吸附(ELISA)法测定大鼠血清中E2的含量,免疫组化即用二步法观察各组大鼠左股骨髁关节骨组织中TGF-β1的分布,采用图像分析仪比较TGF-β1在骨微量环境中积分光密度值(IOD值),将结果进行统计学分析。结果:OP模型组SD大鼠膝关节软骨退变程度较对照组明显加重,骨小梁局部区域变细,排列较紊乱,出现较多的断裂点。股骨近端骨密度较对照组明显下降,差异有统计学意义(P<0.05)。OP模型组血清中E2的含量较对照组明显降低,骨组织中TGF-β1的表达明显低于对照组,差异有统计学意义(P<0.05～0.01)。结论:TGF-β1和E2在绝经后骨质疏松症中起重要调节作用。

Objective： To establish and improve osteoporosis （OP） animal model, observe the expression of serum estrogen and articular bone tissue of transforming growth factor β1 （TGF-β1） on OP model, and study the relationships between them. Methods： Twenty 4-month-old female SD rats were divided randomly into blank control group, OP model group （removal of bilateral ovarian）, 10 in each group. Two months later the rats were sacrificed, took the right proximal femur using dual-energy X-ray bone mineral density scanner to detect, the left femoral condyle articular bone tissue morphology was tested by light microscopy observation, EIASA method was used to measure serum E2 levels by immunohistochemical method of transforming growth factor-β1 distribution in the rat left femoral condyle articular bone tissue. Image analysis was used to compare TGF-β1 in the bone micro-environment of the integral optical density （IOD values）. Results： The degree of knee joint cartilage degeneration in OP model was heavier than that in the control group. In the OP model, trabecular bone thinning local area, with more disorders, occured more frequently the breaking point, and proximal femoral bone mineral density was loner than the control group the difference had statistical significance （P 〈0.05）. In OP model group, serum E2 levels was significantly lower than the control group, bone tissue expression of TGF-β1 was significantly lower than the control group, the difference had statistical significance （P 〈0.05）. Conclusion： TGF-β1 and E2 play an important regulatory role on postmenopausal osteoporosis.