橙皮苷治疗大鼠非酒精性脂肪肝的研究

Experimental study of effect of hesperidin treatment on rat non-alcoholic fatty liver disease

目的:研究橙皮苷对大鼠非酒精性脂肪肝(NAFLD)的治疗作用。方法:给予大鼠高脂饲料以建立NAFLD模型,随机分为正常对照组、模型组、橙皮苷(40、80、160mg/kg)组和烟酸组(120mg/kg),实验结束后检测血脂水平、转氨酶活性、血清和肝匀浆MDA、SOD水平,同时取肝组织进行病理学检查。结果:橙皮苷能明显降低NAFLD大鼠血脂水平和转氨酶活性,显著降低NAFLD大鼠过高的MDA水平,升高其低下的SOD水平,并可以改善肝细胞脂肪变性、降低肝脏炎症反应。结论:橙皮苷对NAFLD大鼠有明显的治疗作用,其机制可能与抗脂质过氧化有关。

To study the effects of hesperidin treatment on rat non-alcoholic fatty liver disease （NALFD）. Methods： The NAFLD rat model was established by giving rat high-fat forage. The rats were randomly divided into six groups： a normal control group, a model group, hesperidin （40, 80 and 160 mg/kg） groups and a nicotinic acid （ 120 mg/kg） group. After experiment, the levels of aminopherase, blood lipids, MDA and SOD of serum or liver homogenate were detected. Meanwhile, the pathological changes of liver organ were observed. Resuits： Compared to NALFD rats, the levels of aminopherase and blood lipids were obviously decreased after hesperidin treatment. In addition, hesperdin could reduce the elevated MDA level and improve the decreased SOD level of serum or liver homogenate. Further more, hesperidin could ameliorate hepatocellular steatosis and depress liver inflammation. Conclusion： Hesperidin has significant therapeutical effect on NAFLD rat and its mechanism is possibly related to its anti-lipid peroxidation properties.