人参皂苷Rg3对小鼠艾氏腹水癌的抑制作用

Effect of Ginsenoside Rg3 in the treatment on Ehrlich ascites carcinoma in mice

目的:研究人参皂苷Rg3对小鼠艾氏腹水癌的抑制作用及其相关机制。方法:建立昆明种小鼠艾氏腹水癌模型24小时开始用药,人参皂苷Rg3组采用灌胃给药20mg/kg.d,顺铂组0.5mg/kg.d和生理盐水组采用腹腔注射,每日1次,连续2周。治疗结束后24小时,处死各组半数小鼠测各项指标,剩余小鼠停止治疗并观察生存时间。结果:各用药组较生理盐水组在腹水量、腹水中瘤细胞数和瘤细胞存活率方面都下降。免疫组化结果显示:人参皂苷Rg3组的nm23和CD44的表达率分别为90%和70%,与生理盐水组比较均有统计学意义,MMP-2的表达率为80%,与生理盐水组比较无差异。人参皂苷Rg3组和顺铂组较生理盐水组小鼠寿命延长。结论:人参皂苷Rg3对小鼠恶性腹腔积液的形成及癌细胞有抑制作用,并可以延长其生存时间,其机制可能与调节nm23和CD44的表达有关。

To observe the effect of Ginsenoside Rg3 on mice Ehrlich ascites carcinoma cells and its related mechanism. Methods： sixty mice were randomized into three groups ： normal saline group （2ml/d） ; csplatin group （0.5mg/kg. d ） ; Ginsenoside Rg3 group （20mg/k9. d） ; All groups were established experimental Ehrlich ascites carcinoma lines model, and 24 hours later intraperitoneal infusion of medicines was given to each mouse once every day for 2 weeks, Ginsenoside Rg3 group were garage administration, cisplatin group and the saline group intraperitoneal injection drug use. Results： Amount of ascites, number of tumor cells and survival rate of tumor cells of csplatin group and Ginsenoside Rg3 group were less than normal saline group（ P 〈0.05 ）. Immunohistochemistry test results show： Ginsenoside Rg3 group nm23 and CIM-4 expression rates were 90% and 70%, with the saline group were statistically significant. MMP-2, the rate of 80% , with the saline group was not significant. Ginsenoside Rg3 group and cisplatin group than extend the life of mice with normal saline group. Conclusion： Ginsenoside Rg3 inhibited formation of malignant ascites and carcinoma cells and may extend survival time in mice. The mechanism may be related to regulating the expression of CD44 and nm23.