黄芪总苷对兔动脉粥样硬化斑块中平滑肌表达的影响

Effects of astragalosides on smooth muscle in rabbit atherosclerotic plaques

目的:观察黄芪总苷对兔动脉粥样硬化斑块胶原含量的影响,并对其机制进行初步探讨。方法:高胆固醇饮食12周建立兔动脉粥样硬化(atherosclerosis,AS)病变模型,黄芪总苷10、30、90mg/kg·d灌胃。取主动脉内膜组织分别采用western、免疫组织化学方法检测斑块组织中Ras p21蛋白、SMCα-actin、c-jun、c-fos、c-myc蛋白含量。结果:黄芪总苷能明显降低斑块中SMCα-actin、Ras p21蛋白,c-jun、c-fos、c-myc蛋白和mRNA表达。结论:黄芪总苷能降低动脉粥样硬化斑块中平滑肌含量,其机制可能与降低Ras、c-jun、c-fos、c-myc表达有关。

Rabbit atherosclerotic models were set up to study effects of astragalosides on smooth muscle in atherosclerotic plaques and initial investigation for mechanism. Method： Rabbit atherosclerotic models were set up by hypercholestermia diet. Contents of Ras p21, SMC α-actin, c-jun, c-fos, c-myc protein were detected by western blot and immunohistochemical methods, respectively, contents of SMC α-actin, c-jun, c-fos, c-myc protein were measured by Image Pro Plus 4.5. Expression levels of c-jun, c-fos and c-myc mRNA were determined with real-time PCR. Result ： In astragalosides groups, contents of Ras p21 and SMC α-actin protein, c-jun, c-fos, c-myc protein and mRNA were decreased markedly. Conclusion： astragalosides could decrease smooth muscle in rabbit atherosclerotic plaque. This maybe be concerned with that it could decrease expression of Ras, c-jun, c-fos and c-myc.