摘要
目的:探讨胞嘧啶脱氨酶(CD)基因联合5-氟胞嘧啶(5-FC)热化疗对裸鼠结肠癌肝转移的治疗效果。方法:30只BALB/c裸鼠经门静脉注射人结肠癌Lovo细胞建立结肠癌肝转移模型,随机分为转基因组和非转基因组,每组15只。转基因组:经腹腔注射病毒上清(0.2mL/d,共5d)进行原位基因转染;非转基因组经腹腔注射生理盐水(0.2mL/d,共5d),2组同时腹腔注射42℃500mg·kg-1.d-1)5-FC。治疗21d后将动物处死,观察肝脏转移率和转移结节数,PCR法检测目的基因在肿瘤组织的整合,RT-PCR法检测目的基因在肿瘤组织的表达,观察肿瘤病理学变化。结果:目的基因成功转染并在肿瘤组织中有效表达。转基因组与非转移基因组肝脏转移率分别为26.7%(4/15)、100.0%(15/15),2组比较差异有统计学意义(P<0.05),肝脏转移结节数分别为0.60±0.83、2.40±0.99(P<0.001)。镜下转基因组肝内转移癌细胞变性,染色体消失,可见凋亡小体,而非转基因组未见明显变化。结论:原位基因治疗联合前药热化疗对裸鼠结肠癌肝转移有明显抑制作用。
Objective: To investigate the treatment effect of cytosine deaminase gene combined 5-FC thermochemotherapy for colon cancer liver metastasis in Nude Mice. Methods: The models of liver metastasis of colon cancer had been established by injecting human colon cancer cells Lovo into portal vein in 30 BALB/c nude mice,which were divided into transgenic group and non-transgenic group randomly. Transgenic group:virs supernatant was injected into abdominal cavity(0.2 mL/d,for 5 days); nontransgen c group: sodium chloride was injected into abdominal cavity(0.2mL/d,for 5days).In both groups 42℃5-FC was injected into the abdominal cavity(500mg·kg^-1·d^-1,for 21days).After treatment,all mice were sacrificed ,and the PCR and RT-PCR were paerformed to detect the expression of targeted gene in carcinoma.The pathology changes of tumor were observed.Resultes:The targeted gene were detected in the rumor tissues.The rates of liver metastasis tumors were 26.7%(4/15),100.0%(15/15) in transgenic group and non-transgenic group respectively(P〈0.05),the number of liver metastasis nodules were 0.60±.83,2.40±0.99respectively (P〈0.001).In transgenic group,liver metastasis cell denatured,and chromosome disappeared.Conclusions:Cytosine deaminase gene combined 5-FC thermochemotherapy has and obvious inhibiting effect on the growth of liver metastasis of colon cancer in nude mice.
出处
《中国现代普通外科进展》
CAS
2009年第7期553-556,共4页
Chinese Journal of Current Advances in General Surgery
基金
福建省自然科学基金资助项目(No.C0410045)