荧光原位杂交技术在法庭科学DNA检验中的应用

Applications of Fluorescence in Situ Hybridization(FISH) in Forensic DNA Analysis

目的运用荧光原位杂交技术结合激光显微切割技术,分离法医物证男女混合样本中的男性细胞和女性细胞,并进行DNA分型。方法通过双色荧光原位杂交,Y染色体标记上绿色信号,X染色体标记上红色信号,在荧光显微镜下识别男性细胞和女性细胞,并通过激光显微切割技术分别获得男性细胞和女性细胞进行DNA分型。结果运用荧光原位杂交技术,能够分别标记法医物证男女混合样本中的男性细胞和女性细胞,并通过激光显微切割技术获得各自的DNA分型。结论荧光原位杂交技术结合激光显微切割技术,可应用于法医物证男女混合样本的检验,提高个体识别能力。

Objective To prove the reliability for sex identification by detection of chromosome X and Y using fluorescence in situ hybridization and to obtain individuate DNA profiles with laser capture microdissection （LCM） from male/female mixture. Methods After fluorescence in situ hybridization, both fluorescence red signal X and green signal Y was found under a fluorescence microscope from male cells and the successful red signal of two X was observed from female cells, Combined with laser capture microdissection, DNA could be individually obtained. Results Using fluorescence in situ hybridization, male cells and female cells could be differently stained. Short tandem repeat profiling was performed after isolation of the detected cells with laser capture microdissection. Conclusion Fluorescence in situ hybridization combined with laser capture microdissection could provided a new useful method for forensic DNA analysis in male/female mixture.