摘要
目的建立HPLC测定盐酸异丙嗪片中盐酸畀丙嗪含量的方法。方法采用Agilent Eclipse XDB-C18色谱柱,流动相:乙腈-1.2%庚烷磺酸钠溶液(磷酸调节pH=2.5)(45:55,v/v),柱温:28℃,检测波:249nm,流速:1.0mL·min^-1,进样量:10μL。结果盐酸异丙嗪在0.0611~0.6108μg与峰面积呈良好的线性关系(r=0.9997);平均回收率为99.8%,RSD=0.92%(n=6)。结论本方法简便、准确、重现性好,可用于该制剂的质量控制。
Objective To establish an HPLC method for the determination of promethazine hydrochloride in promethazine hydrochloride tablets. Methods An Agilent Eclipse XDB-C18 chromatographic column was used with acetonitrile- 1.2% sodium heptanesulfonate (adjust pH to 2.5) by phosphoric acid 45 : 55 (v/v) as the mobile phase at 28 ℃. The detection wavelength was 249 nm. The flow rate was 1.0 mL .min^-1. Results The linear range was 0. 061 1-0. 610 8 vg (r=0. 999 7). The average recovery was 99. 8%, and RSD:0. 92G (n=6). Conclusion The method is simple, accurate and reproducible. It can be used in the quality control of this preparation.
出处
《中南药学》
CAS
2009年第8期603-605,共3页
Central South Pharmacy
关键词
HPLC
盐酸异丙嗪
含量测定
HPLC
promethazine hydrochloride
content determination.