乙酰胆碱酯酶在不同品系和不同性别淡色库蚊的变化

The changes of acetylcholinesterase in different strains and gender of Culex pipiens pallens

目的探讨乙酰胆碱酯酶(acetylcholinesterase,AChE)在不同品系和不同性别淡色库蚊的变化,为应用生物化学方法检测蚊虫抗药性提供依据。方法以碘化硫代乙酰胆碱为底物,5,5′-二硫-双(2-硝基苯甲酸)为显色剂,测定单个蚊虫AChE,以残杀威为抑制剂测定AChE不敏感性。结果抗DDVP(Rd)和抗残杀威(Rp)品系淡色库蚊AChE活性水平高于敏感(S)品系,抗氯氰菊酯(Rc)品系淡色库蚊AChE活性与S品系相近。Rd、Rp品系淡色库蚊不敏感AChE个体频率高于S品系,Rc品系淡色库蚊不敏感AChE个体频率与S品系相近。S、Rd、Rp、Rc4个品系淡色库蚊3日龄雌雄成虫AChE活性均是雌性大于雄性。结论测定AChE可用来判断蚊虫抗药性状况,不同性别蚊虫应分别制定判断标准。作者简介:李士根(1966-),男,山东人,博士,副教授,主要从事医学寄生虫学教学与研究。E-mail:shigenli@yahoo.com.

Objective Study the changes of acetylcholinesterase （ACHE） activity in different strains of adult male and female Culex pipiens pallerts, and provide a basis for the insecticide-resistance detection of mosquitoes by biochemical method. Methods The AChE activity of single mosquito was determined, and acetythiocholine iodide （ATch） as the substrate and 5, 5＇ - dithio-bis （2-nitrobenzoic acid） （DTNB） as the developer. The insensitivity of AChE to Propoxur was assayed. Results The AChE activity of anti-DDVP （Rd） and anti-Propoxur （Rp） strains of Cx.pipiens pallens was significantly higher than that of susceptible （S） strains, however, the AChE activity of anti-Cypermethrin （Rc） strains was similar to that of S strain. The individual frequency of insensitive AChE of Rd strain and Rp strain was significantly higher than that of S strain, but that of Rc strains was similar to S strain. For S, Rd, Rp and Rc strains, the AChE activity of 3-day-old female mosquito was higher than that of 3-day-old male mosquito. Conclusion The resistance of mosquitoes could be judged by the determination of AChE activity, but a criterion should be different for the male and female mosquitoes.