乙型肝炎病毒在乙型肝炎病毒表面抗原阴性模式者血液中的检测情况分析

Detection of HBV by PCR in HBsAg negative people

目的了解HBsAg阴性模式时乙型肝炎病毒在体内存在情况，评价其临床意义和聚合酶链反应方法检测乙型肝炎病毒的应用价值。方法以HBsAg阴性模式者为对象，采用酶联免疫吸附法检测血清乙型肝炎病毒血清标志物；生化检测血清丙氨酸转氨酶、总胆红素、总蛋白和凝血酶原活动度；用聚合酶链方法检测乙型肝炎病毒DNA。结果282例HBsAg阴性者，血液乙型肝炎病毒阳性26例（9．2％）。这26例患者中，24例丙氨酸转氨酶升高，16例总胆红素升高，11例凝血酶原活动度〈70％。结论各种乙型肝炎标志物模式时体内都可能存在乙型肝炎病毒，此时应做检测乙型肝炎病毒确证试验。聚合酶链反应方法可能是目前临床最实用的检测乙型肝炎病毒的确证实验方法。

Objective To observe the clinical value of detection of hepatitis B virus （HBV） by polymerase chain reaction（PCR） in HBsAg negative people. Methods Object of the study was HBsAg negative people. The HBsAg,Anti-HBe, HBeAg, Anti-HBe, Anti-HBc and Anti-HBc IgM were detected by enzyme-linked immunoabsorbent assay. The HBV was detected by PCR. Results Among two hundred and eighty-two HBsAg negative patients, positive rate of serum HBV was 9.2%. Twenty-four cases of them had a high level of alanine aminotransferase, 16 cases had a high total bilirubin, and prothrombin time activity in 11 cases was less than 70%. Conclusion HBV could exist in blood in any model of hepatitis B markers and needs validation test. PCR is the most useful method for definitive detection of HBV.