乳腺癌组织中脆性组氨酸三联体基因的表达及意义

Expression and significance of gene fragile histidine triad in breast carcinoma

目的探讨乳腺癌组织中脆性组氨酸三联体（FHIT）基因的表达及其临床意义。方法应用免疫组织化学酶联免疫sP法，检测FHIT在41例乳腺癌组织及11例癌旁组织中的表达。结果乳腺癌组FHIT蛋白阳性表达率46．3％（19／41）显著低于正常乳腺组90．9％（10／11）（P〈0．05）。中／高分化组FHIT蛋白阳性表达率显著高于低分化组（53．8％vs33．3％）（P〈0．05）；淋巴结转移组FHIT蛋白阳性率低于，无淋巴结转移组（42．9％vs53．8％）（P〈0．05）；Ⅰ＋Ⅱ期组与Ⅲ＋Ⅳ期组之间FHIT蛋白阳性表达率（56．3％ vs 40．0％）差异有统计学意义（P〈0．05）。结论FHIT基因在乳腺癌的发生、发展中起重要作用，对其蛋白的检测可作为判定乳腺癌发生及转移能力的一项客观指标。

Objective To investigate the expression and significancof fragile histidine triad（FHIT） gene in breast carcinoma. Methods The expression of FHIT was detected by immunohistochemistry SP method in 41 cases of breast carcinoma and 11 of adjacent normal breast tissue. Results 46.3 % （ 19 of 41 ） of cases had expression of FHIT in the tumor cells and 90.9 percent （ 10 of 11 ） of cases of the normal tissue were FHIT positive. There was a significant positive correlation between breast carcinoma specimens and normal tissue specimens （ P 〈 0.05 ）. The positive rates of FHIT in high and middle degrees of differentiation were significantly higher than that in low degree of differentiation（53.8% vs 33.3% ）. Tumors with lymph node metastases（42.9% ） had loss FHIT protein expression than those without metastasis （53.8%） （ P 〈 0.05 ）. The FHIT expression between Ⅰ ＋Ⅱ and Ⅲ ＋ Ⅳ stage showed a significant difference （56.3% vs 40.0% ） （ P 〈 0.05 ）. Conclusions FHIT gene plays an important role in the occurrence and development of breast carcinoma. It can be a useful marker for predicting invasion and metas- tasis ability of breast carcinoma.