摘要
为探讨内毒素血症时山羊肝线粒体一氧化氮(NO)及一氧化氮合成酶(NOS)的变化,将72只山羊随机分成6组,每组12只,分别为A组对照组、B组内毒素血症模型组(内毒素,LPS 1 mg/kg体质量)、C组内毒素+褪黑素组(褪黑素,MT1 mg/kg体质量)、D组褪黑素组(MT1 mg/kg体质量)、E组内毒素+氨基胍组(氨基胍,AG25 mg/kg体质量)、F组氨基胍组(AG25 mg/kg体质量)。分别于处理后3 h和6 h各宰杀6只山羊提取肝线粒体,检测肝线粒体中NO、T-NOS和i NOS的变化。结果显示,山羊内毒素血症时肝脏线粒体NO水平升高,NOS活力增强。应用褪黑素和氨基胍治疗后,肝脏线粒体NO水平降低,NOS活力受到抑制,证明2种药物都具有一定的保护作用。
To investigate the effect of nitric oxide (NO) and nitric oxide synthase (NOS) on liver mitochondrial in gaots, 72 goats were divided randomly into 6 groups : A control, B lipopolysaccharide (LPS, 1 mg/kg BW), C lipopolysaccharide+ melatonin ( MT, 1 mg/kg BW), D melatonin ( MT, 1 mg/kg BW), E lipopolysaccharide + aminoguanidin (AG,25 mg/kg BW) and F aminoguanidin (AG,25 mg/kg BW). The liver mitochondrial NO,T-NOS and iNOS were examined at 3^rd h and 6^th after administration,respectively. Results revealed that NO and NOS increased in B group while decreased by the treatment of MT and AG. It is concluded that MT and AG could preserve the liver mitochondria by the inhibition of NO and NOS.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第8期1058-1061,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30371068)
关键词
内毒素
褪黑素
氨基胍
线粒体
一氧化氮
一氧化氮合成酶
lipopolysaccharide
melatonin
aminoguanidin, mitochondria
nitric oxide
nitric oxide synthase