摘要
目的分析探讨HLA-A、HLA-B和HLA-DRBl基因低分辨相合无关供/受者对与HLA高分辨水平匹配的比例和特点。方法对318份HLA-A、B和DRBl基因低分辨相合的无关供/受者对标本进行HLA测序分型,从高分辨水平统计和分析HLA各基因座匹配的比例和特点,以及HLA系统中6个等位基因(HLA-A、HIA-B和HLA-DRB1位点)、8个等位基因(HLA-A、HLA-B、HLA-Cw和HIJA.DRB1位点)、10个等位基因(HLA-A、HLA—B、HIJA-Cw、HLA-DRB1和HLA-DQB1位点)的匹配性。结果单个HLA位点完全相合的比例为HLA-B[79.9%(254/318)]〉HIJA.DRB1[64.8%(206/318)]〉HLA-DQB1[62.3%(134/215)]=HLA-A[62.3%(198/318)]〉HLA-Cw[55.3%(119/215)]。6个等位基因完全相合的比例为36.2%(115/318),8个等位基因完全相合的比例下降为21.9%(47/215),10个等位基因完全相合的比例为20.5%(44/215)。在76份HLA-A、HIJA-B和HLA-DRBl等位基因6/6相合的样本中,等位基因8/8相合及10/10相合的比例分别为63.2%(48/76)和57.9%(44/76);但其中1~2个HLA-Cw等位基因不相合的比例为36.8%(28/76),且主要为抗原水平不相合。结论实现HLA-A、HLA-B、HLA-DRBI的高分辨分型数据入库虽有助于提高无关供/受者对与HLA高分辨配型检索的成功率,但HLA-Cw抗原水平的不相合不能被忽视,建议将HLA-Cw基因分型纳入骨髓库骨髓志愿捐献者HLA入库数据的常规检测。
Objective To analyze and evaluate the high-resolution HLA matching status for the unrelated donor-receipt pairs who were matched at low-resolution for HLA-A, HLA-B and HLA- DRB1. Methods A total of 318 samples from Chinese Marrow Donor Program (CMDP) unrelated donor- receipt pairs matched at low-resolution for HLA-A, HLA-B and HLA-DRB1 were subjected to HLA sequence-based typing (SBT). HLA matching status at each locus, as well as 6 alleles matching status for HLA-A, HLA-B and HLA-DRB1, 8 alleles matching status for HLA-A, HLA-B, HLA-Cw and HLA-DRB1 and 10 alleles matching status for HLA-A, HLA-B, HLA-Cw, HLA-DRB1 and HLA-DQB1 were analyzed. Results The ratio for high-resolution HLA comprehensive matching at each locus were ranked as : HLA-B[79.9% (254/318) ] 〉 HLA-DRB1 [ 64. 8% ( 206/318 ) ] 〉 HLA-DQB1 [ 62. 3% ( 134/215 ) ] = HLA-A[62. 3% (198/318) ]〉 HLA-Cw[55.3% (119/215) ]. The ratio for HLA comprehensive matching at 6 alleles, 8 alleles and 10 alleles were 36. 2% ( 115/318), 21.9% (47/215) and 20. 5% (44/215), respectively. In the 76 unrelated donor-receipt pairs matching at high-resolution for HLA-A, HLA-B and HLA-DRB1, the ratio for donor-receipt pair allele full matching at 8/8 and 10/10 were 63.2% (48/76) and 57.9% (44/76) , respectively. However, 36. 8% (28/76) of them were found to carry single or two HLA- Cw alleles mismatching and predominately showed HLA-Cw antigen level mismatching. Conclusions HLA-A, HLA-B and HLA-DRB1 high-resolution genotyping for unrelated marrow donor will help improving thepercentage of HLA allele full matching for unrelated donor-receipt pairs. However, HLA-Cw mismatching at antigen level could no longer be ignored. The results indicated that HLA-Cw genotyping should be incorporated into future CMDP unrelated marrow donor routine HLA test.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2009年第8期910-914,共5页
Chinese Journal of Laboratory Medicine