TRAP分子标记在棉花海陆渐渗杂交后代选择中的应用研究

Application of TRAP Molecular Marker to the Selection from Post-generation of G.hirsutum and G.Barbadense Introgression Hybridization

利用与棉花纤维伸长相关和纤维细胞次生壁发育相关的基因设计了9条固定引物,与42条随机引物组合形成378对引物。从中筛选出亲本间有显著差异长绒棉共显性TRAP(目标区域扩增多态性)标记12个。用这12个标记对4个海陆杂交组合、每组合40个BC1单株的后代进行了鉴定,用3个标记对另7个海陆杂交组合、每组合40个BC1单株的后代进行了鉴定,发现分离是普遍存在的,在所有检测的BC1代中,平均分离比例接近50%。显示这些长绒棉共显性的标记可以广泛的用于任意海陆杂交或渐渗杂交后代是否携带与标记相关海岛棉基因的选择,不只针对特殊的亲本和一对杂交亲本的分离群体。为实现TRAP分子标记辅助育种与棉花品质性状的遗传改良奠定了基础。

378 pair of primers combined by 9 fixed primers and 42 arbitrary primer, 12 codominance markers of G. Barbadense were screened, which can distinguish G. hirsutum and G. Barbadense. The fixed primers were designed from genes related to fiber elongation and secondary wall development. The 12 eodominance markers were used to analyse 40 BC1 plants from 4 G. hirsuturn and G. barbadense introgression and 3 codominance markers to analyse 40 BCI plants from another 6 G. hirsutum and G. barbadense introgression respectively. Results showed that the separation commonly existed in all BC1 generations and the separation ratio was around 50%. It is suggested that these codominance markers can be extensively used to distinguish whether the post-generation from any of G. hirsutum and G. barbadense introgression carry the gene from G. barbadense or not, not only used to distinguish the specific parents or a post-generation from a pair of parents. TRAP can be used for molecular marker assistant selection and cotton fiber quality improvement in cotton breeding.