大鼠慢性高眼压青光眼模型的构建及鉴定

Construction and appraisal of chronic ocular hypertension glaucoma model of rats

目的:手术建立慢性高眼压青光眼大鼠模型对研究青光眼发病机制、治疗和视神经保护这三方面的研究尤为必要。方法:烧灼3条表浅巩膜静脉,并术中联合应用结膜瓣下浸有0.2g/L的丝裂霉素的凝胶海绵,阻断大鼠的眼部房水外流,建立大鼠慢性高眼压模型,手术后2h;1,3,7,14,28,56d处死5只大鼠,空白对照组亦处死5只大鼠;摘除眼球光镜下进行视网膜形态的观察及视网膜厚度测量,研究眼压、视网膜神经节细胞(retinal ganglion cells,RGC)和视神经损伤的特性。结果:大鼠手术眼与假手术眼、空白对照眼的术后各时间点的眼压比较差异有显著性(P<0.01)。结论:建立大鼠慢性高眼压模型操作简单,重复性好,成模率高,高眼压可稳定维持长时间,慢性高眼压将导致整个视网膜的变薄和萎缩。

AIM： The establishment of chronic ocular hypertension surgery glaucoma animal model for the study of the pathogenesis, treatment and protection of these three aspects of the optic nerve is particularly necessary. METHODS： Superficial burning three episcleral veins, and surgery combined with conjunctival flap under the Baptist has the mitomycin C 0.2g/L gel sponge , to block rat aqueous outflow of the eye can induce by intraocular pressure（lOP）, the establishment of rat model of chronic high intraocular pressure. After surgery 2 hours, 1 day, 3, 7, 14, 28, 56 days, 5 rats were killed, blank control group, also killed 5 rats; Eyeballs were removed for light microscopy, retinal eye patterns observation and measurement of retinal thickness in order to study the lOP, retinal ganglion cells（RGC） and optic nerve injury characteristics. RESULTS： The rats with sham surgery eye eyes, eye control of postoperative intraocular pressure at different time points of comparison there was a significant difference （ P〈 0.01 ）. CONCLUSION： Rats with chronic ocular hypertension model is simple, reproducible and high rate into a mold, high intraocular pressure may maintain stability for a longtime. Chronic high intraocular pressure of the final results will lead to the thinning of the retina and atrophy.