不同卵巢组织冷冻方法对保存女性生育力的研究

Preservation of fertility of female: research of method for human ovarian tissue cryopreservation

目的比较程序化冷冻和玻璃化冷冻两种方法保存人卵巢组织,解冻后组织病理分析原始卵泡存活情况。方法卵巢组织取自15例卵巢畸胎瘤患者,卵巢皮质切割成10 mm×1 mm×1 mm的组织条随机分配到玻璃化冷冻组(A组)、程序化冷冻组(B组)、新鲜对照组(C组),组织病理分析比较三组原始卵泡形态的影响。结果三组共有572个原始卵泡,C组中96.9%的原始卵泡保持形态学正常,A和B组分别为85.6%与83.9%,差别有统计学意义(P<0.01);A与B组比较,差别没有统计学意义(P>0.05)。结论尽管冻融后组织的原始卵泡存活率小于新鲜组织,玻璃化冷冻与程序化冷冻方法都能较好地保存人类卵巢组织,可用于人类卵巢组织的冷冻保存。

Objective：To compare the effect of slow freezing and vitrification protocol for cryopreservation of human ovarian tissues by analyze the morphological integral primordial follicles rates after warming/thawing. Methods：The human ovarian tissues were collected from 15 patients suffered from teratoma. Human ovarian cortex was incised to pieces in size of 10 mm× 1 mm× 1 mm. The tissue pieces were randomly divided into vitrification group （A）, conventional slow freezing group （B） and fresh group （C）. The effect of two cryopreservation protocols on morphological integral primordial follicles was compared by histological analysis. Results：There were 572 primordial follicles in three groups, the morphological integral primordial follicles rates were 96. 9% in group C, 85. 3% in group A, 83. 9% in group B. There was significant difference on normal morphology primordial follicle rates among three groups （P〈0.01）, no significant difference on normal morphology primordial follicle rates were observed between vitrification group （A） and conventional slow freezing group （B） （P〉0.05）. Conclusions：Although the normal follicle percentages in the frozen groups were lower than the fresh group, the programmed cryopreservation and vitrification freezing can both preserve the human ovarian tissues well, so both of them can be adopted.