摘要
以白兰花半木质化的侧芽为外植体,用两种灭菌剂配合使用比单一使用效果要好,污染率从100%下降到20%以下;最佳芽诱导培养基为1/2MS+6-BA1.0mg/L+2,4-D0.1mg/L+KT0.5 mg/L+椰子水5%+蔗糖30 g/L+琼脂5.8 g/L;最佳增殖培养基为MS+6-BA1.0mg/L+NAA0.1mg/L+KT0.1mg/L+GA30.1mg/L+蔗糖6%+琼脂粉5.8g/L。
The disinfection effect was much better by using two kinds of microbicide than one. The contamination rate was lowered from 100% to 20% while disinfecting the semi-lignified lateral buds explants of Michelia alba DC.The best buds induction medium was 1/2MS＋6-BAI.0 mg/L＋2,4-D0.1 mg/L＋KT0.5 mg/L＋coconut water 5%＋cane sugar 30 g/L＋agar 5.8 g/L. The best buds multiplication medium was MS＋6-BA1.0 mg/L＋NAAO.1 mg/L＋KT0.1 mg/L＋GA30.1 mg/L＋ cane sugar 6%＋agar 5.8 g/L.
出处
《广东农业科学》
CAS
CSCD
北大核心
2009年第6期46-49,共4页
Guangdong Agricultural Sciences
关键词
白兰花
灭菌剂
诱导
增殖
Michelia alba DC.
microbicide
induction
multiplication
