基于元分析的大豆生育期QTL的整合

An Integrated QTL Map of Growth Stage in Soybean[Glycine max(L.)Merr.]:Constructed through Meta-Analysis

共搜集整理了12年来已经报道的与大豆生育期有关的98个QTL,通过BioMercator2.1和公共标记映射整合到大豆公共遗传连锁图谱soymap2上,并利用元分析技术推断QTL位置,计算提取真正有效的QTL。发掘出大豆两个重要生育时期,共9个"真实QTL"及其连锁标记,其中与开花期(R1)相关的有7个,与成熟期(R8)相关的有2个,建立了QTL的一致性图谱,其中L连锁群上的一个定位区间包含一个已发表的有关R1的基因。在5个连锁群上共发现10个控制多个生育时期的QTL。本研究结果为大豆生育期QTL精细定位和基因克隆奠定了基础。

Soybean is one of the most important crops in the world, which is kept improving for its yield and quality. Growth stage is a critical trait in soybean development and production which is one of the quantitive traits depending on many loci. As far as the technology of QTL comes, quantitive traits mapping has becoming hot point. Located the QTL controlling soybean growth stage by genetic linkage, is very useful to molecular breeding and deeper understand the process of growth stage develop. Till now, a lot of QTLs related with soybean growth stage were mapped, but many pseudo-positive QTLs were included. To mining the true and major QTLs, meta-analysis were introduced in this study. According to the map of soybean soymap2 published in 2004, an integrated QTL map of soybean growth stages was constructed. The QTLs of soybean growth stage were collected in recent 12 years, and projected to the reference map from their own maps by the software BioMercator2.1. In total, 98 QTLs related with different growth stage of soybean were integrated, including the QTLs of vegetative growth and reproductive growth. A method of meta-analysis was used to narrow down the confidence interval. Seven R1 real QTLs and two R8 real QTLs as well as their corresponding markers were obtained respectively, and a known gene was found in a mapping interval in LG L, located on 93.26 cM. The shortest confidence interval is only 0.9 cM in LG C2, with the marker A397_1 on the left and the marker Satt263 on the right. And 10 QTLs in 5 linkage groups, including C2, D1a, D1b, F, and J, were related to several growth stages. In the combined analysis, a QTL on 55.89 cM in LG D1a controls 6 growth stages, which were R1, R2, R3, R4, R5, and R7. Another QTL on LG D1b, near the markers Bng047_1 and Sat_169, were partly related not only in vegetative growth but also in reproductive growth. The results offer a basis for gene mining and molecular breeding in soybean.