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拟南芥IQM3基因的表达分析及其突变体的鉴定 被引量：6

Expression Analysis of Arabidopsis thaliana IQM3 Gene and Identification of iqm3 Mutants
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摘要 对拟南芥(Arabidopsis thaliana)IQM3基因的功能进行了分析。结果表明,推定IQM3的启动子中存在多种光、非生物胁迫和植物激素反应的顺式作用元件,可能参与植物对环境变化的反应。RT-PCR分析表明,IQM3在拟南芥莲座叶、花序叶、茎、花和根中的表达较强,但在荚果中的表达很弱;IQM3基因的T-DNA插入突变体iqm3-1和iqm3-2分别是功能缺失和超表达突变体,对这些突变体的表型分析表明,IQM3基因与种子萌发及幼苗子叶膨大有密切关系。 The function of IQM3 gene from Arabidopsis thaliana was analyzed. The ressults showed that the putative promoter region of IQM3 contained cis-acting regulatory elements involved in response to environmental changes, such as light, phytohormones and abiofie-stress. IQM3 gene strongly expressed in different organs of 6- week-old plant, such as flower, rosette leaf, eauline leaf, stem, and root, but weekly in pods. Mutants iqm3-1 and iqm3-2 inserted T-DNA of IQM3 gene had been found to be loss-of-function and over-expression line, respectively, by RT-PCR analysis. Phenotypie characterization of the mutants indicated that IQM3 gene related with seed germination and cotyledon expansion.
作者 周玉萍 赵军 何滔 程惠贞 段俊 田长恩
机构地区 中国科学院华南植物园 中国科学院研究生院 广州市植物抗逆基因功能研究重点实验室 广州大学生命科学学院
出处 《热带亚热带植物学报》 CAS CSCD 北大核心 2009年第4期365-370,共6页 Journal of Tropical and Subtropical Botany
基金 广州大学科研创新团队项目(2008)资助
关键词 拟南芥 基因表达 IQM3 iqm3-1 iqm3-2 Arabidopsi thaliana Gene expression IQM3 iqm3-1 iqm3-2
分类号 Q943.2 [生物学—植物学]
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参考文献9

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二级参考文献62

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共引文献51

同被引文献40

  • 1毛国红,宋林霞,孙大业.植物钙调素结合蛋白研究进展[J].植物生理与分子生物学学报,2004,30(5):481-488. 被引量：48
  • 2韦慧彦,郭振清,崔素娟.钙不依赖性钙调素结合蛋白的研究进展[J].生物化学与生物物理进展,2007,34(2):124-131. 被引量：17
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  • 5O' Day DH. CaMBOT : profiling and characterizing calmodulin- binding protein [ J ] . Cell Signal, 2003, 15 : 347-354.
  • 6Rhoads AR, Freiedberg F. Sequence motif and calmodulin recognition [ J ] . FASEB J, 1997, 11 : 331-340.
  • 7Jurado LA, Choekalingam PS, Jarrett HW. Apocalmodulin [ J ] . Physiol Rev, 1999, 79 ( 3 ) : 661-682.
  • 8Abel S, Savchenko T, Levy M. Genome-wide comparative analysis of the IQD gene families in Arabbidopsis thaliana and Oryza sativa [ J ] . BMC Evol Biol, 2005, 5 : 72-89.
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引证文献6

二级引证文献22

热带亚热带植物学报
2009年 第4期

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