P27^(kip1)与细胞周期蛋白G在急性白血病中的表达及相关性研究

Expression of P27^(kip1) and Cyclin G in Patients with Acute Leukemia and Its Correlation

为了探讨P27kip1与细胞周期蛋白G(cyclin G)在急性白血病患者中的表达及其相关性,采用逆转录聚合酶链反应(RT-PCR)检测89例急性白血病患者和10例正常人白细胞的P27kip1与cyclin G mRNA水平,采用Western blot检测39例急性白血病患者和10例正常人白细胞的P27kip1与cyclin G蛋白表达水平。结果显示:急性白血病初治/复发组的cyclin G mRNA与蛋白表达水平均明显高于缓解组和正常对照组(p<0.05或p<0.01);缓解组与正常对照组之间无显著差异(p>0.05)。急性白血病初治/复发组的P27kip1 mRNA水平与缓解组和正常对照组比较均无明显差异(p>0.05)。而急性白血病缓解组和正常对照组的P27kip1的蛋白表达水平高于初治/复发组(p<0.05),缓解组与正常对照组之间无显著差异(p>0.05)。P27kip1与cyclin G的表达呈低度负相关。结论:P27kip1与cyclinG在白血病患者中有异常表达,与白血病的发生发展有一定联系;P27kip1的低表达与cyclin G的异常高表达可能是白血病预后不良的因素之一。

This study was purposed to explore the expression of P27^kip1 and cyclin G in patients with acute leukemia （AL） and its correlation. The reverse polymerase chain reaction（RT-PCR） was used to analyse the expression of P27^kip1 and cyclin G mRNA in 89 AL patients and 10 normal persons; Western blot was used to analyze the expression of P27^kip1 and cyclin G protein in 39 AL patients and 10 normal persons. The results showed that the cyclin G mRNA and protein expressions in new diagnosed/relapsed cases of AL were significantly higher than those in patients with remission and normal controls （p 〈0.05 and p 〈0.01 ）, but there was no difference between remission cases and normal controls（p 〉 0.05 ）. The expression of P27^kip1 mRNA in newly diagnosed/relapsed patients with AL was not significantly different from patients with remission and normal controls （p 〉 0.05 ）, while the P27^kip1 protein expression in remission cases of AL and normal controls was significantly higher than that in new diagnosed/relapsed cases（p 〈 0.05 ）, but there was no difference between remission cases and normal controls （p 〉 0.05 ）. The expression of P27^kip1 negatively and lowly correlated with the expression of cyclin G in patients with AL. It is concluded that the low expression of P27^kip1 and the high expression of cyclin G in patients with AL may have some correlation with genesis and development of AL and may be an indication for poor prognosis of AL.