活化的T细胞促进间充质干细胞分化为成骨细胞

Activated T Cells Promote Differentiation of Mesenchymal Stem Cells into Osteoblasts

本研究旨在探讨活化的T细胞对间充质干细胞(mesenchymal stem cell,MSC)向成骨细胞分化的影响。将活化的T细胞与MSC共培养,14天后碱性磷酸酶染色,观察成骨细胞的生成。此外,在MSC的培养体系中加入活化的T细胞上清,RT-PCR检测活化的T细胞免疫相关分子的表达,探讨哪些细胞因子参与了MSC向成骨细胞分化。结果表明,活化的T细胞能促进MSC分化为成骨细胞,IL-1β在其中发挥重要的作用,而TNF-α,TGF-β1作用不明显。结论:活化的T细胞促进MSC向成骨细胞分化,MSC与免疫细胞通过细胞因子发生相互作用。

This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1β played an important role in the effect of activated T cells on MSCs, while TNF-α, TGF-1β were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interatcive influence between MSCs and immune cells can be mediated through cytokines.