摘要
本研究旨在探讨活化的T细胞对间充质干细胞(mesenchymal stem cell,MSC)向成骨细胞分化的影响。将活化的T细胞与MSC共培养,14天后碱性磷酸酶染色,观察成骨细胞的生成。此外,在MSC的培养体系中加入活化的T细胞上清,RT-PCR检测活化的T细胞免疫相关分子的表达,探讨哪些细胞因子参与了MSC向成骨细胞分化。结果表明,活化的T细胞能促进MSC分化为成骨细胞,IL-1β在其中发挥重要的作用,而TNF-α,TGF-β1作用不明显。结论:活化的T细胞促进MSC向成骨细胞分化,MSC与免疫细胞通过细胞因子发生相互作用。
This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1β played an important role in the effect of activated T cells on MSCs, while TNF-α, TGF-1β were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interatcive influence between MSCs and immune cells can be mediated through cytokines.
出处
《中国实验血液学杂志》
CAS
CSCD
2009年第4期974-976,共3页
Journal of Experimental Hematology
基金
973国家重点基础研究资助项目(编号2005CB522705)
863国家高新技术研究发展项目(编号2007AA021109)
国家自然科学基金项目(编号30600309
30730043)