广州管圆线虫感染致大鼠肺组织病理改变及免疫组化研究

Immunohistochemical and Pathological Study of the Lung of Rat Infected by Angiostrongylus cantonensis

目的观察动物感染广州管圆线虫后肺组织病理改变,以及应用抗广州管圆线虫成虫单克隆抗体进行感染鼠肺免疫组织化学研究。方法20只大鼠实验室人工感染广州管圆线虫,组织学方法观察肺组织的形态学变化,免疫组织化学方法应用实验室制备的抗广州管圆线虫成虫可溶性抗原单克隆抗体IgG和IgM进行肺组织切片的免疫反应性研究。结果受感染的大鼠肺组织肿胀实变,表面及切面质硬粗糙,有灰白色虫卵结节,病变范围广泛。HE染色镜下观察可见肺组织中有多个圆形、椭圆形的虫卵结节,结节周围组织细胞反应及纤维化,肺泡隔增厚,肺泡轮廓消失。结节内虫卵发育形成桑葚期、仔虫期及一期幼虫。用抗广州管圆线虫成虫单克隆抗体IgG和IgM分别行免疫组化,结果显示桑葚期虫卵表达IgG,而IgM则在桑葚期、仔虫期及一期幼虫等部位均有显著阳性表达。结论广州管圆线虫感染所致大鼠肺脏病理改变,主要表现在肺内形成多个虫卵结节,并可致肺呈纤维化改变;抗广州管圆线虫成虫单克隆抗体IgG和IgM在肺组织虫卵结节内的阳性表达有显著差别。

Objective To study the pathological changes and immunohistochemical study of the lung of rat infected by Anglostrongylus cantonensis. Method 20 rats were infected with Angiostrongylus cantonensis, and then observe the morphological damages and the immunoreactivity in the lung by histological methods and immunochemical analysis using monoclonal antibody IgG/IgM against adult worm of Anglostrongylus cantonensis. Result Swelling and consolidation of lung were observed in rats infected by Angiostrongylus cantonensis after 6 weeks. The surfaces of lung were stiff and rough with gray worm eggs nodes, with widespread pathological areas. Many spherical or elliptical worm eggs nodes had been observed under light microscope by hematoxylin-eosin staining, with fibrous degeneration around the nodes. The interalveolar septum thickening and the alveolus circumscription disappeared. Worm eggs in the nodes were developed to form morulas, worms and first stage larvas. Results of immunohistochemical staining with monoclonal antibody IgG/IgM against adult worm of Anglostrongylus cantonensis indicated the immunoreactivity antigens of IgG were mainly located in the worm eggs of morula, but the antigens of IgM were expressed in all locations of morula, worms and first stage larvas. Conclusion The main pathological chamge in lung of rat infected with Anglostrongylus cantonensis is a lot of worm eggs nodes forming together with fibrous degeneration in lung. The expressions of immunoreactivity antigens in different developmental stages of eggs nodes are different significantly with the staining of monoclonal antibody IgG/IgM against adult worm of A nglostrongylus cantonensis.