电针对犬幽门括约肌压力的影响及作用机制

Effects of Electroacupuncture on the Pressure in Pylonic Antrum in Dogs

目的观察电针(electroacupuncture,EA)对犬幽门括约肌压力的调控作用及其与血浆、胃粘膜组织中降钙素基因相关肽(calcitonin gene-related peptide,CGRP)、内皮素(endothelin,ET)含量变化的关系,探讨电针调控胃功能的作用机制。方法将20只犬随机分为4组:空白对照组、非经非穴组、上巨虚组、足三里组(每组5只)。采用胃压测量仪监测犬幽门括约肌压力的变化,同步测定血浆及胃粘膜组织中CGRP、ET含量,并观察变化规律。结果电针足三里穴后幽门扩约肌总压力、基础压(P<0.01)、频率下降(P<0.05),血浆及胃粘膜组织中CGRP含量上升(P<0.01),ET含量显著下降(P<0.01),上巨虚穴组幽门扩约肌总压力、基础压下降,仅血浆CGRP(P<0.05)含量上升,内皮素含量下降,但足三里组变化趋势更明显,其他组各监测指标无显著变化。结论电针可使犬幽门括约肌压力变化,对部分胃功能具有调控作用,并与影响某些脑肠肽的含量改变有关,具有一定的经络和穴位特异性。

Objective To investigate the regulation of electroacupuncture （EA） on the pressure in pyloric antrum and its relation to the contents of calcitonin gene-related peptide （CGRP） and endothelin （ET） in plasma and gastric mucosa in dogs. Methods Twenty dogs were randomly and evenly divided into 4 groups, i.e. control group, Feijingfeixue group, Zusanli group and Shangjuxu group. By using manometric recording technique and radioactive-immunon-assay methods, the pressure in pyloric sphincter and the contents of calcitonin gene-related peptide and endothelin in plasma and gastric mucosa were simultaneously measured. Results In Zusanli group, after EA stimulation, a decrease was observed in the total pressure, basic pressure （P〈0. 01）, wave frequency （P〈0. 05）, contents of ET in plasma and in gastric mucosa, whereas an increase was observed in CGRP （P〈0. 01 ） as compared with those of the control group. In Shangjuxu group, after EA stimulation, a decrease in the total and basic pressures and ET content in plasma, and an increase in plasma CGRP content （P〈0. 05） were observed. The change in Zusanli group was more significant than Shangjuxu group. No significant changes were observed in other groups. Conclusion Electroacupuncture at Zusanli point can significantly reduce pressure in pyloric sphincter and has retulative effect on gastrointestinal functions. This acupoint specific effect is probably related to the changes of brain-gut peptide.