小鼠小肠缺血再灌注损伤后FGFR3基因的变化

Change of FGFR3 after Intestinal Mucosal Ischemia-reperfusion Injury in Mice

目的通过检测成纤维细胞生长因子受体3(fibroblast growthfactor receptor3,FGFR3)在小鼠缺血再灌注(I/R)损伤后肠上皮基因表达变化,为进一步研究FGFR3对缺血再灌注损伤的修复作用及机制奠定基础。方法采用小鼠肠系膜上动脉夹闭法制备缺血1h后再灌注动物模型。分为正常组、野生鼠I/R组和FGFR3增强小鼠I/R组。检测再灌注1、3、6h和1、3d时肠上皮损伤程度和血浆D-乳酸水平,用实时逆转录-聚合酶联反应(Realti me-PCR)检测小肠中FGFR3mRNA表达。结果肠缺血再灌注1、3、6h,肠粘膜损害明显,肠粘膜再生1、3h,肠粘膜结构逐渐恢复至正常。FGFR3增强小鼠在各时间点肠粘膜损害程度均轻于野生小鼠。两组小鼠缺血再灌注损伤后D-乳酸水平在各时相点均明显高于正常对照组小鼠,并且均在再灌注1h后达到峰值,然后逐渐下降。但FGFR3增强小鼠在再灌注1、3、6h,均显著低于野生小鼠。两组小鼠缺血再灌注损伤后FGFR3mRNA表达明显增高,均在再灌注1h后达到第一个峰值,然后迅速下降,于3h达到低谷,再迅速升高达到第二个峰值后逐渐下降,3d仍处于较高水平。结论缺血再灌注损伤后小鼠小肠中FGFR3mRNA表达量显著增加。

Objective To explore the change of fibroblast growth factor receptor 3 （FGFR3） in small intestine after ischemia/reperfusion （I/R） injury in mice. Methods Superior mesenteric artery （SMA） was occluded to produce ischemia of the intestine for i hour followed by reperfusion to reproduce I/R injury. Mice were divided into normal group, wild I/R group and FGFR3^＋ I/R group. The damage degree of small intestinal epithelium and plasmal D-lactic acid levels were determined 1 hour, 3 hours, 6 hours, 1 day and 3 days after reperfusion, mRNA expression level of FGFP,3 in small intestine were determined by reverse transcription-polymerase chain reaction （Realtime-PCR） assay. Results Progressively severe mucosal injury occurred 1, 3, 6 hours after reperfusion of the ischemic intestine. At the 1, 3 day timepoint, the recovery of mucosal injury was observed, and the damage in small intestinal epithelium was significantly lower in FGFR3^＋ I/R group than wild I/R group. The D-lactic acid level of FGFR3^＋ I/R and wild I/R groups were remarkably higher than in normal group at every timepoint. They both reached peak 1 hour after reperfusion, then gradually decreased, but the level was significantly lower in FGFR3^＋ I/R group than wild I/R group 1, 3, 6 hours after reperfusion, The mRNA expression level of FGFR3 in small intestine of the two groups were remarkably elevated and reached the first peak 1 hour after reperfusion, quickly decreased to the ebb 3 hours after reperfusion, rose again significantly to the second peak and then gradually decreased, while they remained at a high level at 3 day timepoint. Conclusion Expression level of FGFR3 mRNA in small intestine increased significantly in mice with I/R injury.