姜黄胶囊治疗实验性大鼠糖尿病足的研究

Study of Turmeric capsule on experimental rats with diabetic foot

目的观察姜黄胶囊(CC)对糖尿病足(DF)大鼠模型空腹血糖(FBG)、空腹血清胰岛素(FSI)、血流变(CBF)及足部溃疡症状的影响。方法选用SPF级健康雄性Wistar大鼠60只,随机抽取10只作为正常对照组,其余50只制作糖尿病足模型,47只造模成功,按随机数字表法分为姜黄胶囊高剂量治疗组(CH组,16只)、中剂量治疗组(CM组,11只)、低剂量治疗组(CL组,10只)和糖尿病足空白对照组(DFB组,10只);正常对照组大鼠仅建立足溃疡模型。造模后对各组大鼠进行药物干预,于腹主动脉采血,测定FBG、FSI、CBF。结果CH、CM组FBG水平较CL组明显降低(P均<0.05);CH、CM组FSI较CL组明显升高(P均<0.05);血流变中低切变率、血浆黏度、红细胞压积、红细胞聚集指数及红细胞变形指数CH、CM组与CL组比较存在显著性差异(P均<0.05);3组治疗后的体质量、血流变中的高切变率及足部症状评分比较均无显著性差异(P>0.05)。结论姜黄胶囊对糖尿病足大鼠具有较好的降低血糖、升高空腹血清胰岛素、改善糖尿病足大鼠的血流变和局部血液循环的作用,姜黄胶囊治疗糖尿病足的作用机制与姜黄胶囊降低血糖、改善血液流变学情况有关。

Objective It is to observe the influence of turmeric capsule （CC） on fasting blood glucose （FBG）, fasting serum insulin （FSI）, blood flow changes （CBF） and the impact of foot ulcer symptoms of rats with diabetic foot （DF）. Methods 60 SPF - class healthy male Wistar rats were selected, and randomly selected 10 cases as normal control group, and 47 of the remaining 50 rats were made DF model successfully, and randomly divided into CC high-dose group （CHG, 16 rats）, middle-doses group （CMG, 11 rats）, low-dose group （CLG, 10 rats） and DF blank control group （DFBG, 10 rats）; the foot ulcers were made only in normal control group. The drug intervention was executed, then the blood was collected from the abdominal aorta to measure FBG, FSI, CBF. Results The levels of FBG in CHG and CMG was significantly lower than CLG （ P 〈 0.05） ; and the levels of FSI in CHG and CMG was significantly higher than CLG （P 〈 0.05 ） ; there is significant difference in blood flow change in the low shear rate, plasma viscosity, hematocrit, erythrocyte aggregation index and RBC deformability index of in CHG and CMG compared with CLG （P〈0.05）. There was no significant difference in the body weight, blood flow changes and foot symptom score in the high shear rate among three groups after treatment（ P 〉 0.05）. Conclusion CC has better action to lower FBG, elevated FSI and improves blood flow in rats with DF and change the role of local blood circulation. The mechanism of CC in the treatment of DF is in a relationship with the situation of lower blood sugar and improves hemorrheology.