罗格列酮对急性胰腺炎模型大鼠NF-κB与TNF-α表达的影响

Effects of Rosiglitazone on the Expression of NF-κB and TNF-α in Rats with Acute Pancreatitis

目的观察罗格列酮对大鼠急性胰腺炎(AP)的疗效和对核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)表达的影响。方法大鼠胆胰管逆行注射5%牛磺胆酸钠制备AP模型,治疗组在造模前30min股静脉注射罗格列酮6mg·kg-1。观察胰腺组织病理学改变并评分,检测血清淀粉酶(AMY)和组织髓过氧化物酶(MPO)水平;免疫组化检测NF-κB表达变化,逆转录聚合酶链反应(RT-PCR)检测TNF-α mRNA的表达。结果罗格列酮治疗组胰腺病理变化评分低于模型组,血清AMY和组织MPO水平亦低于模型组,NF-κB和TNF-α mRNA表达较模型组减弱(均P<0.01)。结论罗格列酮可缓解AP大鼠胰腺的损伤,其机制与抑制胰腺NF-κB和TNF-α表达有关。

Objective To study effects of rosiglitazone on the expression of NF-κB and TNF-α in rats with acute pancreatitis. Methods Acute pancreatitis model was induced by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct. Rosiglitazone （6 mg · kg^-1 ） was injected by femoral vein 30 minutes prior to the operation. Serum amylase （ AMY）, tissue myeloperoxidase （ MPO ） level and pancreatic pathologic score were detected. The expression of nuclear fatorkappa B （NF-κB） in pancreatic tissue was assayed by immunohistochemistry and TNF-α mRNA was analyzed by reverse transcription polymerase chain reaction （PCR）. Results Compared with the model group, the levels of amylase, MPO, and pathologic score in the rosiglitazone treated group were reduced （ P 〈 0.01 ） , both the expression of NF-κB and TNF-α were decreased （P 〈0.01 ）. Conclusion Rosiglitazone exerts protective effects on acute pancreatitis, which could be associated with down-regulation of NF-κB and TNF-α exoression.