荧光法研究β-CD/HP-β-CD存在时甲基蓝与人血清白蛋白的相互作用

Fluorescence Study on the Interaction between Methyl Blue and Human Serum Albumin in the Presence of β-CD/HP-β-CD

用紫外-可见及荧光光谱法研究了有无环糊精(CDs)条件下甲基蓝(MB)与人血清白蛋白(HSA)的相互作用,详细讨论了pH值的影响。实验结果表明,HSA可以增强MB的荧光;CDs的存在可以使MB进入CDs的疏水空腔使得从S1到S0的辐射跃迁得到保护,从而增强MB的荧光;同时CDs可以通过改变MB的微环境形成MB-CDs包合物而增强MB与HSA的相互作用。根据Benesi-Hildebrand方程计算出二元超分子体系MB-HSA、MB-β-CD/HP-β-CD和三元超分子体系MB-β-CD/HP-β-CD-HSA的结合比及结合常数。

The interaction between methyl blue （MB） and human serum albumin （HSA） in the presence or absence of β-cyclodextrin （β-CD/HP-β-CD） was studied based on the UV-Vis and fluorescence methods. Experimental factor of pH value was discussed in detail. The experiment results showed that the fluorescence of MB can be enhanced by HSA, and the fluorescence of MB can be further enhanced in the presence of CDs because MB can enter the hydrophobic cavity of CDs and the radiant transition from S1 to S0 is protected. Meanwhile, CDs can strengthen the binding interaction between MB and HSA by changing the microenvironment of MB and forming MB-CDs supramolecular system. The inclusion ratio and inclusion constants of MB-HSA, MB-β-CD/HP-β CD and MB-β-CD/HP-β-CD-HSA were calculated according to Benesi-Hildebrand equation by fluorescence spectrometry.