超高效液相色谱-串联质谱法同时测定食品中碱性橙、碱性嫩黄O和碱性桃红T

Simultaneous Determination of Chrysoidine,Auramine O and Safranine T in Foods by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

本文建立了同时测定食品中碱性橙、碱性嫩黄O和碱性桃红的超高效液相色谱-串联质谱（UPLC～MS／MS）检测方法。样品经碱化甲醇提取、二氯甲烷萃取、浓缩、酸化甲醇溶解和甲醇饱和正己烷溶液萃取净化后进行测定。本方法检出限分别为碱性橙0．6μg／kg、碱性嫩黄O0．3μg／kg、碱性桃红T0．7μg／kg。六种食品样品的回收率为72．1％～91．1％，相对标准偏差（RSD）为2．4％～9．1％（n=6），三种染料在0．01～0．5μg／mL范围内呈良好的线性关系，线性回归系数r均大于0．9950。

A method for simultaneous determination of chrysoidine, auramine O and safranine T in foods by ultra performance liquid chromatography-tandem mass spectrometry has been developed. The food samples were extracted by basified methanol followed by extraction with CH2Cl2 and concentration. The concentrated mixtures were re-dissolved in acidified methanol and cleaned up with hexane. Ultra performance liquid chromatography-tradem mass spectrometry （UPLC-MS/MS） was used to quantify three dyes. The linear range were 0.01-0.5 μg/mL（r=0. 995）. The recoveries ranged from 74.3% to 91.1%, and the relative standard deviations were 2.4%-9.4% （n=6）. The detection limits were 0.6, 0.3 and 0.7 μg/kg for chrysoidine, auramine O and safranine T, respectively.