摘要
采用双夹心酶联免疫吸附法(DAS-ELISA),对杭州、嘉兴、绍兴3市6县(市、区)的果树园林植物进行李属坏死环斑病毒病的病情调查,采集检测样品包括樱桃、桃、李、杏和月季等12种寄主植物共计102个。结果表明,10个海宁月季样品的ELISA结果呈阳性,其他样品呈阴性;阳性样品经RT-PCR检测和CP基因测序验证,确认海宁月季感染了李属坏死环斑病毒。
A survey on Prunus necrotic ringspot virus disease of plants in Hangzhou, Shaoxing and Jiaxing areas was under- taken during 2007 - 2008. Total 102 samples of twelve fruit and ornamental host plants including cherry, peach, plum, almond, and rose were collected for detection. The results showed that 10 of 14 rose samples from Haining county were DAS- ELISA positive, which was then comfmned by RT-PCR and sequencing of the CP gene. It was concluded that these ten rose samples from Haining County, Zhejiang Province, were infected by Prunus necrotic ringspot virus.
出处
《浙江农业学报》
CSCD
北大核心
2009年第4期366-369,共4页
Acta Agriculturae Zhejiangensis
基金
浙江省重大科技专项(2006C12020)
浙江省教育厅(2005079411)
