磁微粒分离酶联免疫法在日本血吸虫抗体检测中的应用

Detection of serum antibodies from patients with Schistosoma japonicum by magnetic affinity immunoassay

目的探讨磁微粒分离酶联免疫法(MPAIA)用于检测日本血吸虫病血清抗体的效果。方法用MPAIA测定日本血吸虫病血清中特异性抗体。结果用MPAIA检测384例非血吸虫病流行区阴性血清,均为阴性;检测61例急性血吸虫病人血清、788例慢性血吸虫病人血清、32例晚期血吸虫病人血清其阳性率分别为100%、94.2%、90.6%;检测14例并殖吸虫病人、13例囊虫病人、9例旋毛虫病人血清,均未发现交叉反应。结论MPAIA检测日本血吸虫血清抗体有较高的灵敏度和特异度,可用于日本血吸虫病的临床诊断和现场疫情监测,该方法具有广阔应用前景。

To observe the effect of magnetic affinity immunoassay（MPAIA） on detecting the serum antibodies of patients with Schistosoma japonicum, this method of assay was used to detect specific serum antibodies of 384 patients with Schistosoma japonicum. It was found that the false positive rate of 384 sera of healthy persons from non-endemic region was 0. And the positive rate of 61 sera from acute schistosomiasis patients, 788 sera from chronic schistosomiasis patients and 32 sera from advanced schistosomiasis patients were 100%, 94.2% and 90.6 %, respectively. The cross reaction rate of sera from patients infected with other parasites（14 patients with paragonimiasis, 13 patients with cysticercosis and 9 patients with trichinosis） was 0. The result shows that MPAIA is a sensitive and specific assay to detect serum antibodies from patients with schistosomiasis and MPAIA can be applied to detect the serum antibodies from patients infected with Schistosoma japonicum.