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免疫组织化学法和逆转录聚合酶链反应法检测胃癌微转移的研究 被引量:2

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摘要 目的评价免疫组织化学(IHC)和逆转录聚合酶链反应法(RT-PCR)对胃癌淋巴结微ΚΣ的临床意义应⒚。方法选择2002年4月至2008年7月我院施行的61例胃癌根治术中切除的淋巴结标本,在传统病理检查的基础上,用IHC法和RT-PCR法同步检查细胞角蛋白20(CK20)和癌胚抗原(CEA)的表达。结果淋巴结阳性检出率由传统的HE染色的70.6%分别上升至82.7%和89.2%,IHC和RT-PCR法检测重新分期率分别为21.31%和32.79%。结论IHC和RT-PCR法是检测胃癌淋巴结转移的有效手段,为准确分期并制定相应的治疗方案提供了依据。
出处 《中华普通外科学文献(电子版)》 2009年第4期42-43,共2页 Chinese Archives of General Surgery(Electronic Edition)
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