黄柏生物碱成分在二妙丸类方中保护尿酸盐致人血管内皮细胞损伤作用及其配伍变化的研究

Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula

研究对二妙丸类方(二妙丸、三妙丸、四妙丸和加味四妙丸)起重要作用的基本方二妙丸的物质基础及其配伍变化.正交组合二妙丸类方组方药有效部位,四氮唑盐(MTT)法评价优选出对保护尿酸钠结晶(MSU)致人血管内皮细胞(HUVEC)损伤起重要作用的二妙丸黄柏生物碱部位,HPLC/DVD法建立黄柏生物碱部位指纹图谱,HPLC-ESI-MS/MS分析共有峰成分,鉴定了12个生物碱成分,并比较了二妙丸类方有效部位群HPLC/DVD指纹图谱配伍类方前后黄柏生物碱共有峰相对峰面积比值的变化.结果表明,黄柏生物碱配伍二妙丸及其类方后总相对含量分别为122.9%,80.7%,60.2%和94.2%,小檗碱、巴马汀等重要生物碱也呈现较大的变化趋势,茵芋碱、5-羟基小檗碱和四氢巴马汀等成分配伍二妙丸类方后消失.研究显示,基本方二妙丸中的黄柏生物碱是二妙丸类方中保护HUVEC起重要作用的物质基础,其相对含量变化与配伍类方后的功效变化一致.

To reveal the compatibility of traditional Chinese medicine, the material foundation playing major role of Ermiao Wan categorized formula and their compatibility content variation was studied. The activity of the combined effective components by orthogonal design in its relevant herbs were evaluated for protecting HUVEC damaged model induced by urate, the results indicated that the alkaloids components of Phellodendron chinense（PC） of Ermiao Wan play a major role in its categorized formula. The fingerprint of alkaloids compo- nents in PC and Ermiao Wan categorized formula were analyzed and compared by high performance liquid chromatography-electrospray ionization-mass spectrometry（HPLC-ESI-MS）. Twelve alkaloids were detected in the fingerprint of alkaloids. The contents of some alkaloids were found to be reduced, on the contrary, the contents of total alkaloids were increased 122. 9% in Ermiao Wan, and, reduced 80. 7%, 60. 2% , 94.2% in its categorized formula. From the chemical point of view, the material foundation of basic formula plays major role in its categorized formula, the variation of relative content of alkaloids are in accord with the virtue of Ermiao Wan categorized formula.